Upregulation of cell proliferation via Shc and ERK1/2 MAPK signaling in SaOS-2 osteoblasts grown on magnesium alloy surface coating with tricalcium phosphate

Journal of Materials Science. Materials in Medicine
Tianlong JiangYuyan Zhao

Abstract

Magnesium (Mg) alloys have been demonstrated to be viable orthopedic implants because of mechanical and biocompatible properties similar to natural bone. In order to improve its osteogenic properties, a porous β-tricalcium phosphate (β-TCP) was coated on the Mg-3AI-1Zn alloy by alkali-heat treatment technique. The human bone-derived cells (SaOS-2) were cultured on (β-TCP)-Mg-3AI-1Zn in vitro, and the osteoblast response, the morphology and the elements on this alloy surface were investigated. Also, the regulation of key intracellular signalling proteins was investigated in the SaOS-2 cells cultured on alloy surface. The results from scanning electron microscope and immunofluorescence staining demonstrated that (β-TCP)-Mg-3AI-1Zn induced significant osteogenesis. SaOS-2 cell proliferation was improved by β-TCP coating. Moreover, the (β-TCP)-Mg-3AI-1Zn surface induced activation of key intracellular signalling proteins in SaOS-2 cells. We observed an enhanced activation of Src homology and collagen (Shc), a common point of integration between bone morphogenetic protein 2, and the Ras/mitogen-activated protein kinase (MAPK) pathway. ERK1/2 MAP kinase activation was also upregulated, suggesting a role in mediating osteoblastic cell...Continue Reading

References

Jul 31, 2003·Cell·Carsten SchmitzAndrew M Scharenberg
Jul 28, 2006·The International Journal of Biochemistry & Cell Biology·L ZhaoT Zhu
Aug 1, 2007·Journal of Materials Science. Materials in Medicine·Liping XuKe Yang
Jan 11, 2008·The Journal of Biological Chemistry·Kellye C KirkbrideGerard C Blobe
May 8, 2008·Journal of Materials Science. Materials in Medicine·Christoph HesseJens Götze
Jun 5, 2008·Journal of Biomedical Materials Research. Part a·Chaoyuan WangHala Zreiqat
Aug 23, 2008·Dental Materials : Official Publication of the Academy of Dental Materials·Triantafillos D Papadopoulos, Konstantin D Spyropoulos
Sep 23, 2008·Dental Materials : Official Publication of the Academy of Dental Materials·Jiawei WangJan de Boer
Nov 15, 2008·Journal of Cellular Biochemistry·Chun-Han HouChih-Hsin Tang
Nov 27, 2008·Journal of Materials Science. Materials in Medicine·Liping XuKe Yang
Sep 22, 2009·Nature Cell Biology·Tomohiko MurakamiKazunori Imaizumi
Mar 30, 2010·Journal of the Mechanical Behavior of Biomedical Materials·J A ToqueI Sopyan
Aug 21, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·M I BakerJ E Lemons
Sep 10, 2010·The Journal of Biological Chemistry·Yanbin ZhengStephen X Skapek
Feb 11, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Byung-Gyu KimJe-Yoel Cho
Sep 20, 2011·Journal of Cellular Biochemistry·Stefano Zanotti, Ernesto Canalis
Oct 4, 2011·Bone·Kate SullivanAaron Schindeler
Nov 2, 2011·Acta Biomaterialia·Shaylin Shadanbaz, George J Dias
Nov 3, 2011·Journal of Biomedical Materials Research. Part a·Hongwei ChaiKe Yang
Dec 23, 2011·Journal of Materials Science. Materials in Medicine·Nicholas T KirklandMark P Staiger
Dec 23, 2011·Journal of Materials Science. Materials in Medicine·E AghionS Ovadia
Jan 3, 2012·The Journal of Nutritional Biochemistry·Marzia LeidiJeanette A M Maier
Feb 22, 2012·Journal of the Mechanical Behavior of Biomedical Materials·Xiaobo ZhangWenjiang Ding
May 29, 2012·The Journal of Prosthetic Dentistry·Mari KoikeToru Okabe
Jul 18, 2012·Journal of Materials Science. Materials in Medicine·Costantino Del GaudioGiampiero Montesperelli

❮ Previous
Next ❯

Citations

Jan 1, 2017·Stem Cell Research & Therapy·Lekshmi KrishnaDebashish Das
Dec 9, 2020·Journal of Biomaterials Applications·Xiao HanPing Yang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.