Upregulation of centromere protein K is crucial for lung adenocarcinoma cell viability and invasion.

Advances in Clinical and Experimental Medicine : Official Organ Wroclaw Medical University
Yongbo WangYunxia Xiu

Abstract

Identification of functional genes or biomarkers may be helpful for developing new treatment strategies in lung adenocarcinoma (LUAD). The centromere protein K (CENPK) gene has been discovered to be overexpressed and could influence tumor progression in several tumor types. However, its role in LUAD has never been revealed. The purpose of the current study was to detect the effects of CENPK and its mechanisms in the progression of LUAD. Data from The Cancer Genome Atlas (TCGA) and Oncomine databases was used to analyze the expression of CENPK. The relationship between CENPK expression and the prognosis of LUAD was investigated using Kaplan-Meier and Cox regression analyses. The cell viability was monitored with Cell Counting Kit-8 (CCK-8) and colony forming assays, while migration and invasion were analyzed with a transwell assay. The effect of CENPK on the expression of epithelial-mesenchymal transition (EMT) markers were estimated using western blotting. CENPK was significantly overexpressed in LUAD tissues and cells (p < 0.01). The overall survival rate in the low CENPK expression group was significantly higher than in the high CENPK expression group (p = 0.003). Furthermore, the overexpression of CENPK facilitated cell viab...Continue Reading

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