Upregulation of dNTP Levels After Telomerase Inactivation Influences Telomerase-Independent Telomere Maintenance Pathway Choice in Saccharomyces cerevisiae

G3 : Genes - Genomes - Genetics
Paula M van MourikMichael Chang

Abstract

In 10-15% of cancers, telomere length is maintained by a telomerase-independent, recombination-mediated pathway called alternative lengthening of telomeres (ALT). ALT mechanisms were first seen, and have been best studied, in telomerase-null Saccharomyces cerevisiae cells called "survivors". There are two main types of survivors. Type I survivors amplify Y' subtelomeric elements while type II survivors, similar to the majority of human ALT cells, amplify the terminal telomeric repeats. Both types of survivors require Rad52, a key homologous recombination protein, and Pol32, a non-essential subunit of DNA polymerase δ. A number of additional proteins have been reported to be important for either type I or type II survivor formation, but it is still unclear how these two pathways maintain telomeres. In this study, we performed a genome-wide screen to identify novel genes that are important for the formation of type II ALT-like survivors. We identified 23 genes that disrupt type II survivor formation when deleted. 17 of these genes had not been previously reported to do so. Several of these genes (DUN1, CCR4, and MOT2) are known to be involved in the regulation of dNTP levels. We find that dNTP levels are elevated early after telo...Continue Reading

References

Feb 24, 1989·Cell·B J Thomas, R Rothstein
Jul 12, 1984·Nature·J ShampayE H Blackburn
Oct 21, 1994·Science·M S Singer, D E Gottschling
Dec 14, 1999·The Journal of Biological Chemistry·A ChabesL Thelander
Mar 7, 2001·Current Biology : CB·P HuangE J Louis
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Xiaolan Zhao, Rodney Rothstein
Jun 21, 2002·Methods in Enzymology·Fred Sherman
Jul 26, 2002·Nature·Guri GiaeverMark Johnston
Nov 22, 2002·Human Molecular Genetics·Dimitrios J StavropoulosM Stephen Meyn
Mar 13, 2003·Molecular Biology of the Cell·Arne S IJpma, Carol W Greider
Dec 4, 2003·Molecular and Cellular Biology·Nathalie Grandin, Michel Charbonneau
May 6, 2004·Genetics·Laura Maringele, David Lydall
Oct 29, 2004·Molecular and Cellular Biology·Anthony J Cesare, Jack D Griffith
May 3, 2005·The Journal of Biological Chemistry·Yong-Bin ChenJin-Qiu Zhou
May 19, 2005·Molecular and Cellular Biology·Janet R MullenSteven J Brill
Aug 25, 2005·Methods in Molecular Biology·Amy Hin Yan Tong, Charles Boone
Mar 21, 2006·The Journal of Biological Chemistry·Hung-Ji TsaiShu-Chun Teng
Jun 13, 2006·Nature Cell Biology·Michel Larrivée, Raymund J Wellinger
Dec 13, 2006·Journal of Cell Science·Robert N WoolstencroftMike Tyers
Jul 20, 2007·Molecular and Cellular Biology·Brietta L Pike, Jörg Heierhorst
Dec 14, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·K R Straatman, E J Louis
Feb 12, 2008·Current Protocols in Molecular Biology·D A Treco, V Lundblad
Sep 23, 2008·Nature·Eleni P Mimitou, Lorraine S Symington
Oct 15, 2008·Molecular Cell·Yang David LeeStephen J Elledge
Mar 24, 2010·Cell Research·Fei-Long MengJin-Qiu Zhou
Nov 5, 2010·Annual Review of Genetics·Devanshi Jain, Julia Promisel Cooper
Feb 25, 2011·PLoS Biology·Angela DeemAnna Malkova
Mar 8, 2011·Cell·Douglas Hanahan, Robert A Weinberg

❮ Previous
Next ❯

Citations

Nov 10, 2021·G3 : Genes - Genomes - Genetics·Enikő Fekete-SzücsMichael Chang
Jan 20, 2022·ELife·Fernando Rodrigo Rosas BringasMichael Chang

❮ Previous
Next ❯

Methods Mentioned

BETA
SGA
dissection

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.