Upregulation of haeme oxygenase-1 by zinc in HCT-116 cells

Free Radical Research
Abigail F Smith, George Loo

Abstract

Haeme oxygenase-1 (HO-1) is often viewed as a cytoprotective gene. Toxic heavy metals induce HO-1, but it is unclear whether particular metal micronutrients also induce HO-1. Hence, the ability of exogenously-added copper, iron and zinc to influence HO-1 expression in HCT-116 cells was evaluated. Under the chosen experimental conditions, only zinc noticeably increased the expression of HO-1 mRNA and protein. Concurrently, zinc decreased non-protein thiol levels to a certain extent, but zinc did not increase the production of reactive oxygen species (ROS). Moreover, ascorbate and Trolox did not inhibit zinc-induced HO-1 upregulation. In contrast, deferoxamine blunted the induction of HO-1 mRNA, protein, and enzymatic activity caused by zinc. Additionally, N-acetylcysteine and Tiron inhibited zinc-induced HO-1 upregulation and also nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2). Collectively, these findings suggest that zinc at above normal levels upregulates HO-1 expression in HCT-116 cells in a ROS-independent manner.

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Citations

Jun 12, 2013·Free Radical Biology & Medicine·Weidong WuJames M Samet
Jan 4, 2020·Environmental Science and Pollution Research International·Mediha SefiNejla Soudani
May 23, 2015·American Journal of Physiology. Cell Physiology·Yunfang ChenBing Li
Jan 14, 2017·Inflammopharmacology·Magdalena JaroszTadeusz Librowski

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