Upregulation of HIF-1α protects neuroblastoma cells from hypoxia-induced apoptosis in a RhoA-dependent manner

Molecular Medicine Reports
Cui-Ping YinShou-Wei Yue

Abstract

Hypoxic conditions regulate several metabolic enzymes and transcription factors that are involved in cancer, ischemia and pulmonary diseases. The Ras homolog (Rho) family, including Rho member A (RhoA), is involved in reorganization of the actin cytoskeleton, cell migration and in the regulation of apoptosis and gene transcription. The aim of the present study was to investigate the expression of hypoxia‑inducible factor (HIF)‑α and the activity of RhoA in PC12 neuroblastoma cells under hypoxic conditions. The upregulation of HIF‑α and RhoA by hypoxia was determined using reverse transcription‑quantitative polymerase chain reaction and western blot assays, cell apoptosis was analyzed using flow cytometry, and the activity of caspase 3 was examined using a western blot assay and caspase 3 activity assay kit. The PC12 cells were induced to apoptosis following exposure to hypoxia, and exhibited increased expression of HIF‑α and increased mRNA and protein expression levels of RhoA. The overexpression of HIF‑α attenuated the hypoxia‑induced apoptosis of the PC12 cells. In addition, RhoA knockdown using small interfering RNA abrogated the antagonism of HIF‑1α towards hypoxia‑induced apoptosis. The results of the present study confirm...Continue Reading

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Citations

Mar 31, 2019·International Journal of Molecular Sciences·Viviana CostaGianluca Giavaresi
Feb 6, 2016·Molecular Medicine Reports·Guanglei YangYanfang Hu
Dec 22, 2019·International Journal of Molecular Sciences·Carlos Huertas-CastañoFrancisco M Vega
Jan 13, 2018·International Journal of Oncology·Hayat ZaatitiTamara J Abou-Antoun

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