Upregulation of Id-1 via BMP-2 receptors induces reactive oxygen species in podocytes

American Journal of Physiology. Renal Physiology
Gregor PacheMartin Johannes Bek

Abstract

Bone morphogenetic proteins (BMPs) are secreted signaling molecules, which play a major role in kidney development and disease. Here, we show the existence of mRNA for BMP-2 and for the BMP receptors BMPR1A, BMPR1B, BMPRII, ACVR1A, ACVR2, and ACVR2B in differentiated mouse podocytes and the protein expression of BMPR1A in human glomerular podocytes. BMP-2 dose dependently increases the free cytosolic Ca(2+) concentration in podocytes proving the existence of a functional receptor in these cells. Recent data indicate that in a myoblastic cell line and in a breast cancer cell line, BMP-2 increases the expression of Id-1, a negative regulator of basic helix-loop-helix transcription factors, but the role of BMP-2 stimulated Id-1 expression in the kidney has not been further characterized. Here, we show that BMP-2 increases the expression of Id-1 in differentiated podocytes. To investigate a role of Id-1 for podocyte function, overexpression of Id-1 was induced in differentiated mouse podocytes. Id-1-overexpressing podocytes show an increased NADPH-dependent production of reactive oxygen species (ROS). This effect can be evoked by BMP-2 and can be antagonized by anti-Id-1 antisense oligonucleotides. The data indicate that BMP-2 may,...Continue Reading

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Citations

Apr 2, 2011·The Journal of Biological Chemistry·Fang HaoJennifer D Black
Sep 21, 2011·PloS One·Eric W BrunskillS Steven Potter
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Mar 1, 2011·Experimental and Therapeutic Medicine·Jin-Soon SuhByoung-Soo Cho
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Aug 29, 2018·International Journal of Molecular Sciences·Adam Andruska, Edda Spiekerkoetter
Apr 8, 2020·ACS Pharmacology & Translational Science·Nimna PereraMitchel Tate

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