Abstract
The human salivary gland (HSG) cell line has so far been used as in vitro models for study of the influence of cytokines and pharmacologic agents on salivary glands, as well as a model system for inflammation in Sjögren's syndrome (SS). This study aimed to determine the effect of IL-17 on IL-6 production and the underlying molecular mechanism regulated by the HSG cell line. Immunofluorescence analyses, RT-PCR, and Western blot were conducted to evaluate the IL-17 receptor (IL-17R) expression in cultured HSG cells. Real-time PCR and ELISA were then utilized to establish the mRNA and protein levels of IL-6 in IL-17-stimulated HSG cells. Western blot, flow cytometry, immunofluorescence, and inhibitor analyses were conducted to elucidate the involved signaling pathways. The HSG cells reliably expressed the IL-17R mRNA and its encoded surface-bound protein. The expression of IL-6 mRNA and protein was upregulated by stimulation of HSG cells with IL-17; this effect was impeded by IL-17- or IL-17R-neutralizing antibodies. IL-17 stimulation ended up with the fast phosphorylation of p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK), Akt, and translocation of nuclear factor-kappaB (NF-κB) in the HSG ...Continue Reading
References
Aug 1, 1981·Cancer·K ShirasunaT Miyazaki
Aug 1, 1993·The Clinical Investigator·L GraeveP C Heinrich
Jul 22, 1999·Archives of Oral Biology·P J DanielsY Wang
Oct 11, 2001·Arthritis and Rheumatism·Y KatzY Beer
Apr 23, 2002·Clinical and Experimental Immunology·A KehlenJ Langner
Jan 8, 2004·Cellular Signalling·Judith SylvesterMuhammad Zafarullah
Oct 4, 2005·Nature Immunology·Laurie E HarringtonCasey T Weaver
Oct 9, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Dolores M CortezBysani Chandrasekar
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fei HuangReen Wu
Nov 14, 2007·Journal of Leukocyte Biology·Sara J Molesworth-KenyonRobert N Lausch
Jul 11, 2008·Immunological Reviews·Laura A TesmerDavid A Fox
Oct 22, 2008·Immunology and Cell Biology·Marievic BulosanSeunghee Cha
Jun 13, 2009·Oral Diseases·N RoescherG G Illei
Jul 11, 2009·Journal of Cellular Physiology·Christopher ChuongSeunghee Cha
Aug 25, 2009·The American Journal of Pathology·Gikas E KatsifisSharon M Wahl
Jan 1, 2010·American Journal of Physiology. Renal Physiology·Masayuki IyodaTadao Akizawa
Mar 17, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lucie RousselSimon Rousseau
Aug 25, 2010·Clinical and Experimental Immunology·H TsuboiT Sumida
Sep 22, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Cuong Q NguyenAmmon B Peck
Dec 15, 2010·Molecular Immunology·Lingxin ZhuBin Peng
Sep 8, 2011·European Journal of Oral Sciences·Naoki TakahashiKazuhisa Yamazaki
Jan 21, 2012·Clinical & Developmental Immunology·Diana MieliauskaiteZygmunt Mackiewicz
Sep 10, 2013·Cytokine·Chunfang GuXiaoxia Li
Mar 25, 2015·Rheumatology·María-José BarreraMaría-Julieta González
May 22, 2016·International Journal of Immunopathology and Pharmacology·Sarah BenchabaneChafia Touil-Boukoffa
Mar 21, 2017·Mediators of Inflammation·Taku KuwabaraTerutaka Kakiuchi
Citations
Oct 9, 2019·Journal of Biochemical and Molecular Toxicology·Dongmei MaoQi Zhang
Feb 25, 2020·Scandinavian Journal of Immunology·Zhikai WanWei Zou
Sep 15, 2020·Biological Chemistry·Yu FujisawaTakeshi Uehara
Jun 18, 2019·Expert Opinion on Investigational Drugs·Sarah PringleFrans G M Kroese
Feb 26, 2019·BioMed Research International·Zhemin XianGuanghai Yan
Aug 11, 2019·Stem Cell Research & Therapy·Jing-Yuan LiHong-Bo Xin
Jul 9, 2021·Frontiers in Pharmacology·Bing'e MaChaoqun Ma