Upregulation of Intestinal Mucin Expression by the Probiotic Bacterium E. coli Nissle 1917

Probiotics and Antimicrobial Proteins
Mohamed M Hafez

Abstract

The probiotic E. coli Nissle 1917 (EcN) has been reported to have various health benefits; however, very little is known about their underlying mechanisms. In this regard, the present study aimed to elucidate the effect of the bacterium on mucin production by intestinal epithelial cells. Incubation of HT-29 cells with EcN lead to a contact time-dependent rise in mRNA levels of the MUC2, MUC3, MUC5AC, and MUC5A. The expression was markedly higher with MUC5AC gene. In most cases, MUC genes expression was more pronounced in polarized cells compared to non-polarized ones. In contrast to MUC3, the basal stimulation of polarized cells brought about markedly higher levels of other tested mucins. Similar but milder results were observed when living EcN was replaced by inactivated bacteria. With exception of MUC3, the conditioned media showed no significant effect on the mRNA level of the tested mucins. The above-mentioned mRNA results were confirmed on protein level using enzyme-linked lectin assay (ELLA) and enzyme-linked immunosorbant assay (ELISA). In contrast to other treatments, basal stimulation of polarized cells showed a growth phase-dependent MUC induction with more prominent effect by stationary-phase bacteria. In contrast to...Continue Reading

References

Aug 1, 1995·Journal of Pediatric Gastroenterology and Nutrition·J P LanghendriesJ Senterre
Mar 15, 1994·Biochemical and Biophysical Research Communications·D R Mack, M A Hollingsworth
May 1, 1994·The Journal of Infectious Diseases·R H YolkenJ F Forstner
Jun 1, 1997·Gut·J M Rhodes
Aug 1, 1997·The American Journal of Clinical Nutrition·E J SchiffrinA Donnet-Hughes
Sep 14, 1999·The American Journal of Physiology·G Hecht
Aug 10, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kazuhiro KohriJay A Nadel
Oct 4, 2002·Microbes and Infection·Sophie NuttenPierre Desreumaux
Dec 10, 2002·Pediatric Surgery International·A F MattarA G Coran
Dec 31, 2002·Biochemical and Biophysical Research Communications·Marilynn A LarsonThomas L McDonald
Mar 6, 2004·Cancer Metastasis Reviews·James C Byrd, Robert S Bresalier
Aug 17, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·E GaudierC Hoebler
Sep 16, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·C Caballero-FrancoK Chadee
Mar 31, 2007·Biochimica Et Biophysica Acta·Prakash RadhakrishnanS Niranjali Devaraj
May 15, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mahmoud El HomsiPascale Plaisancié
Feb 27, 2008·Inflammatory Bowel Diseases·Rim Belharbi KrimiJean-Claude Marie
Aug 6, 2011·BMC Infectious Diseases·Suzanne M GarlandUNKNOWN ProPrems Study Group
Sep 29, 2011·Journal of Dairy Science·J L Baugher, T R Klaenhammer

❮ Previous
Next ❯

Citations

Jun 19, 2013·Best Practice & Research. Clinical Gastroenterology·Corina CeapaRaish Oozeer
Sep 2, 2017·Probiotics and Antimicrobial Proteins·Giorgio La FataM Hasan Mohajeri
Apr 17, 2019·Microorganisms·Charlotte SegersFelice Mastroleo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

American Journal of Physiology. Gastrointestinal and Liver Physiology
Jan-Michel Otte, Daniel K Podolsky
The Laryngoscope
Matthew L UbellJoseph E Kerschner
American Journal of Rhinology
H H JungJ H Park
Biochemical Society Transactions
B J Van KlinkenJ Dekker
© 2022 Meta ULC. All rights reserved