Upregulation of lactate-inducible snail protein suppresses oncogene-mediated senescence through p16INK4a inactivation

Journal of Experimental & Clinical Cancer Research : CR
Xiangrui LiZhihao Wu

Abstract

The preferential use of aerobic glycolysis by tumor cells lead to high accumulation of lactate in tumor microenvironment. Clinical evidence has linked elevated lactate concentration with cancer outcomes. However, the role and molecular mechanisms of lactate in cellular senescence and tumor progression remain elusive. The function of Snail in lactate-induced EMT in lung cancer cells was explored by wound healing assay and cell invasion assay. The qRT-PCR and dual luciferase reporter assay were performed to investigate how lactate regulates Snail expression. The level of TGF-β1 in culture supernatant of cells was measured by ELISA for its correlation with extracellular levels of lactate. Ras activity assay and SA-β-gal activity assay were established to determine the effect of lactate on oncogene-induced senescence in human lung epithelial cells. ChIP assays were conducted to determine the binding of snail to p16INK4a promoter. Two TCGA data sets (TCGA-LUAD and TCGA-LUSC) were used to explore the correlations between SNAI1 and CDKN2A expression. In this study, we showed the invasive and migratory potential of lung cancer cells was significantly enhanced by lactate and was directly linked to snail activity. We also demonstrated th...Continue Reading

References

Mar 1, 1996·Clinical & Experimental Metastasis·R Martínez-ZaguilánM J Hendrix
Dec 17, 2002·Journal of Cell Science·Justin P AnnesDaniel B Rifkin
Sep 20, 2005·Cancer Cell·Susan E MoodyLewis A Chodosh
Feb 28, 2008·Clinical & Experimental Metastasis·Raymond E MoelleringRobert J Gillies
Jul 30, 2008·Cell·Joan Massagué
Mar 12, 2009·Cancer Research·Ian F RobeyRobert J Gillies
Dec 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katrin DietlMarina P Kreutz
Mar 20, 2010·Nature Reviews. Cancer·Daniel A TennantEyal Gottlieb
Oct 11, 2011·Seminars in Cancer Biology·Raya Saab
May 3, 2012·Seminars in Cancer Biology·Aristidis Moustakas, Carl-Henrik Heldin
May 3, 2012·Genes & Development·Chi V Dang
May 5, 2012·Seminars in Cancer Biology·Christina Scheel, Robert A Weinberg
Nov 10, 2012·Experimental Cell Research·Rongzhi ZhaoQinghua Zhou
Feb 11, 2014·Seminars in Cell & Developmental Biology·Daniel B Constam
May 23, 2014·Current Biology : CB·Stephanie A Hills, John F X Diffley
Feb 12, 2015·Molecular Biology Reports·Raymond LucTom C Karagiannis
Mar 18, 2015·The Journal of Cell Biology·Howard DonningerGeoffrey J Clark
Mar 31, 2015·The Cancer Journal·Robert J Gillies, Robert A Gatenby
May 12, 2015·Frontiers in Bioscience (Landmark Edition)·Monica GalloSilvio Naviglio
Nov 3, 2016·Nature Reviews. Clinical Oncology·Ubaldo E Martinez-OutschoornMichael P Lisanti

❮ Previous
Next ❯

Citations

Oct 12, 2018·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hanmin Wang, Juli J Unternaehrer
Oct 2, 2020·Cancers·Atsushi Tanabe, Hiroeki Sahara
Jan 24, 2019·Oxidative Medicine and Cellular Longevity·Su Yeon LeeHo Sung Kang
Apr 28, 2019·International Journal of Molecular Sciences·Hyunkoo KangBuHyun Youn
Jul 13, 2019·Frontiers in Aging Neuroscience·Carolina OsorioAdonis Sfera
Mar 11, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shimin XieXiao Zhu
May 6, 2021·International Journal of Molecular Sciences·Daisy Y ShuMagali Saint-Geniez
Aug 18, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Dun NiuZhizhong Xie
Sep 24, 2021·Journal of Experimental & Clinical Cancer Research : CR·Guizhi JiaBo Tang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
pull-down
transfection
fluorescence microscopy
immunoprecipitation
Assay
electrophoresis
ELISA
transfecting

Software Mentioned

Pro Plus
image J
image
Relative Quantification
SPSS
R

Related Concepts

Related Feeds

Cancer Metabolic Reprogramming (Keystone)

Cancer metabolic reprogramming is important for the rapid growth and proliferation of cancer cells. Cancer cells have the ability to change their metabolic demands depending on their environment, regulated by the activation of oncogenes or loss of tumor suppressor genes. Here is the latest research on cancer metabolic reprogramming.

Cancer Metabolic Reprogramming

Cancer metabolic reprogramming is important for the rapid growth and proliferation of cancer cells. Cancer cells have the ability to change their metabolic demands depending on their environment, regulated by the activation of oncogenes or loss of tumor suppressor genes. Here is the latest research on cancer metabolic reprogramming.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

© 2022 Meta ULC. All rights reserved