PMID: 9179626Feb 1, 1997Paper

Upregulation of lymphoid and renal interferon-gamma mRNA in autoimmune MRL-Fas(lpr) mice with lupus nephritis

Inflammation
X Fan, R P Wuthrich

Abstract

MRL-Fas(lpr) mice develop an aggressive form of autoimmunity, characterized by immune complex-mediated glomerulonephritis and massive expansion of lymphoid tissues. Increased MHC class II expression by macrophages and renal parenchymal cells is a prominent feature of MRL-Fas(lpr) mice. Since interferon-gamma (IFN-gamma) is the major and the most potent inducer of MHC class II molecules it could play a pathogenic role in the disease process in MRL-Fas(lpr). We have analyzed IFN-gamma expression in normal and nephritic MRL-Fas(lpr) mice by examining renal and lymphoid IFN-gamma-specific mRNA production, using reverse transcription-polymerase chain reaction (RT-PCR) and Northern blotting. We detect abundant IFN-gamma mRNA expression in the kidney of nephritic MRL-Fas(lpr) by RT-PCR, whereas normal mice display absent or only very weak expression of this cytokine. By RT-PCR, IFN-gamma mRNA is detectable in normal spleen, but is overexpressed in the enlarged spleen and lymph nodes of MRL-Fas(lpr). Northern blotting using total RNA from tissues confirms abundant IFN-gamma expression in spleen and lymph node of MRL-Fas(lpr). We conclude that enhanced renal IFN-gamma mRNA expression is a prominent feature of MRL-Fas(lpr) lupus nephriti...Continue Reading

Citations

Feb 27, 2004·Autoimmunity·Dwight H KonoArgyrios N Theofilopoulos
Jan 12, 2008·Journal of Pharmacological Sciences·Kae TeramotoKatsuyuki Miura
Dec 24, 2011·Arthritis Research & Therapy·Tamara K Nowling, Gary S Gilkeson
Mar 16, 2011·Clinical and Experimental Immunology·D S Vinay, B S Kwon
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christopher M ReillyGary S Gilkeson
Aug 18, 1999·Immunological Reviews·O T ChanM J Shlomchik
Mar 28, 2003·Journal of the American Society of Nephrology : JASN·Yohei IkezumiDavid J Nikolic-Paterson
Feb 19, 2002·Kidney International·Christopher M ReillyGary S Gilkeson

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