Upregulation of matrix and adhesion molecules induced by controlled topography.

Journal of Materials Science. Materials in Medicine
Kirstie D Andrews, John A Hunt

Abstract

Electrostatic spinning is receiving increasing attention in the field of tissue engineering, due to its ability to produce 3-dimensional, multidirectional, microfibrous scaffolds. These structures are capable of supporting a wide range of cell growth; however, there is little knowledge relating material substrates with specific cellular interactions and responses. The aim of this research was to investigate if electrostatically spun scaffolds, with controlled topographical features, would affect the adhesion mechanisms of contacting cells. A range of electrostatically spun Tecoflex SG-80A polyurethane scaffolds was characterized in terms of inter-fibre separation, fibre diameter, surface roughness, void fraction and fibre orientation. Human embryonic lung fibroblasts and human vein endothelial cells were cultured on these scaffolds for 7, 14, 28 days, and analysed for their expression of extracellular matrix and adhesion molecules using image analysis and laser scanning confocal microscopy. There were significant differences in adhesion mechanisms between scaffolds, cell types and culture periods. Fibroblast-scaffolds were stimulated and oriented to a greater degree, and at earlier cultures, by the controlled topographical feat...Continue Reading

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Citations

Feb 6, 2010·Journal of Materials Science. Materials in Medicine·Nicola DettaFederica Chiellini
Aug 1, 2010·Journal of Nanotechnology in Engineering and Medicine·Riffard Jean-GillesMelinda Larsen
Dec 10, 2008·The Journal of the Acoustical Society of America·Sarah A KlemukIngo R Titze
Mar 6, 2012·Journal of Biomedical Materials Research. Part a·Laura E DickinsonSharon Gerecht
Jun 27, 2009·Journal of Biomedical Materials Research. Part a·Nurdan OzkucurT K Monsees
Aug 14, 2012·Journal of Wound Care·M RipponR White
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Han Tsung LiaoJyh-Ping Chen

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