Upregulation of mesothelial genes in ovarian carcinoma cells is associated with an unfavorable clinical outcome and the promotion of cancer cell adhesion.

Molecular Oncology
Kaire OjasaluRolf Müller

Abstract

A hallmark of ovarian high-grade serous carcinoma (HGSC) is its early and massive peritoneal dissemination via the peritoneal fluid. It is generally believed that tumor cells must breach the mesothelium of peritoneal organs to adhere to the underlying extracellular matrix (ECM) and initiate metastatic growth. However, the molecular mechanisms underlying these processes are only partially understood. Here, we have analyzed 52 matched samples of spheroids and solid tumor masses (suspected primary lesions and metastases) from 10 patients by targeted sequencing of 21 loci previously proposed as targets of HGSC driver mutations. This analysis revealed very similar patterns of genetic alterations in all samples. One exception was FAT3 with a strong enrichment of mutations in metastases compared with presumed primary lesions in two cases. FAT3 is a putative tumor suppressor gene that codes for an atypical cadherin, pointing a potential role in peritoneal dissemination in a subgroup of HGSC patients. By contrast, transcriptome data revealed clear and consistent differences between tumor cell spheroids from ascites and metastatic lesions, which were mirrored by the in vitro adherence of ascites-derived spheroids. The adhesion-induced tr...Continue Reading

References

May 18, 1999·The American Journal of Pathology·K LessanA P Skubitz
Oct 18, 2001·International Journal of Gynecological Pathology : Official Journal of the International Society of Gynecological Pathologists·Q J CaoM Li
Aug 10, 2002·Journal of Cellular and Molecular Medicine·N C Popescu, D B Zimonjic
Jul 24, 2010·The American Journal of Pathology·Ernst Lengyel
Apr 6, 2011·Immunotherapy·Claudia C PrestonKeith L Knutson
Jul 2, 2011·Nature·UNKNOWN Cancer Genome Atlas Research Network
Aug 20, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Silke ReinartzUwe Wagner
Sep 24, 2011·Nature Reviews. Cancer·Sebastian VaughanFrances R Balkwill
Sep 29, 2011·PLoS Biology·Julia SalzmanPatrick O Brown
Nov 9, 2011·Cancer Research·Hagen KulbeFrances R Balkwill
Jan 24, 2012·Gynecologic Oncology·Kathryn P Pennington, Elizabeth M Swisher
Feb 4, 2012·Cancer Discovery·Marcin P IwanickiJoan S Brugge
Apr 25, 2012·Cancer Metastasis Reviews·Katharine L SodekMaurice J Ringuette
Sep 19, 2012·Frontiers in Immunology·Jillian L AstaritaShannon J Turley
Sep 22, 2012·The Journal of Pathology·Mauro CastellarinRobert A Holt
Oct 19, 2012·International Journal of Oncology·Masaru Katoh
Feb 22, 2013·Nature Reviews. Cancer·Emma KippsStan B Kaye
Apr 19, 2013·International Journal of Cancer. Journal International Du Cancer·Walter Blum, Beat Schwaller
Jun 21, 2013·International Journal of Cancer. Journal International Du Cancer·Silke ReinartzRolf Müller
Aug 6, 2013·Immunology Letters·Elke FuertbauerHannes Stockinger
Jan 15, 2014·Journal of Cell Science·Jarama Clucas, Ferran Valderrama
May 9, 2014·Oncotarget·Adrian P WiegmansKum Kum Khanna
Sep 10, 2014·The Journal of Clinical Investigation·Hilary A KennyErnst Lengyel
Mar 11, 2015·Journal of Cellular Biochemistry·Julie DragonArti Shukla

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Methods Mentioned

BETA
flow cytometry
SMA
transfection
GTPase

Software Mentioned

KM Plotter
Variant Studio
SeqCap EZ HyperCap
SeqCap EZ
ExAc
integrative genomics viewer
Python
Enrichment

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