Upregulation of microRNA-370 facilitates the repair of amputated fingers through targeting forkhead box protein O1

Experimental Biology and Medicine
Hongxing ZhangDingjun Hao

Abstract

Angiogenesis is critical to the success of digital replantation. Recent study suggests an important regulatory role of microRNA-370 (miR-370) in ischemia-reperfusion injury. However, its function in digital replantation is poorly understood. In this study, we reported that the expression of miR-370 was upregulated in replantation tissues. miR-370 mimic transfection promoted human umbilical vein endothelial cells (HUVECs) proliferation by regulating the cell cycle and inhibited apoptosis. miR-370 mimic transfection also significantly increased HUVECs migration and induced the formation of capillary-like structures in HUVECs, indicated that miR-370 promoted capillary tube formation in vitro. Furthermore, forkhead box protein O1 (FOXO1) was identified as the functional target of miR-370 by dual-luciferase reporter assay. FOXO1 overexpression vector lacked 3'-UTR together with miR-370 mimic transfection strongly abrogated miR-370-induced cell proliferation and the formation of capillary-like structures in HUVECs. Taken together, our results revealed that the upregulation of miR-370 might facilitate the repair of amputated fingers by regulating angiogenesis through targeting FOXO1. This study provided a potential therapeutic target ...Continue Reading

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Citations

Sep 11, 2018·Antioxidants & Redox Signaling·Damian KloskaAnna Grochot-Przeczek
Oct 22, 2020·Journal of Clinical Medicine·Anthony CannavicciMichael J B Kutryk
Dec 16, 2020·Journal of the American Heart Association·Giacomo FratiSebastiano Sciarretta

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Methods Mentioned

BETA
biopsies
Assay
transfection
PCR
reverse transcription PCR
ELISA
FCS
flow cytometry

Software Mentioned

TargetScan
PicTar
SPSS

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