Abstract
Both MicroRNAs and HMGB1 took part in pathological process of myocardial I/R injury though several signaling pathways. We hypothesized that mircoRNA451 (miR-451), a group of small non-coding RNAs, could improve this injury by inhibiting HMGB1. Male SD rats were randomly distributed into 5 groups and subjected to I/R process. After 24 hours of reperfusion injury, the serum content of CK and LDH, the content of MDA in tissue and activity of SOD were detected; The infarcted areas were defined by TTC staining and Evans Blue; TUNEL staining and cleaved-Caspase 3 were used to test apoptosis; HMGB1 was detected by real-time fluorescence quantitative PCR and Western Blotting. Compared with the I/R and I/R+Ad-GFP group, upregulation of miR-451 could reduce the infarcted areas, cardiomyocytes apoptosis index, expression of cleaved-caspase 3 and content of CK and LDH significantly(P<0.05); Meanwhile, upregulation of miR-451 could also obviously inhibit HMGB1, the increase of MDA and the decrease of SOD (P<0.05). So this study revealed that upregulation of miR-451 could prevent myocardial I/R injury by suppressing HMGB1.
References
Feb 24, 2006·British Journal of Cancer·H-W Hwang, J T Mendell
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Marjorie P Perron, Patrick Provost
Nov 7, 2007·The Journal of Experimental Medicine·Allan TsungTimothy R Billiar
Jan 15, 2008·Cell·Ana EulalioElisa Izaurralde
May 27, 2008·Circulation Journal : Official Journal of the Japanese Circulation Society·Satoshi HagiwaraTakayuki Noguchi
Jun 25, 2008·Circulation·Martin AndrassyAngelika Bierhaus
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eva van RooijEric N Olson
Apr 22, 2009·Circulation·Xiao-Ping RenGuo-Chang Fan
Jun 10, 2009·International Heart Journal·Yehua TangGaozhong Huang
Apr 22, 2010·European Journal of Pharmacology·Xiaorong HuHong Jiang
Jun 3, 2010·Frontiers in Bioscience (Scholar Edition)·Qing-Wu YangJing-Cheng Li
Aug 4, 2010·Genes & Development·Duonan YuMitchell J Weiss
Aug 17, 2010·Journal of Molecular and Cellular Cardiology·Xiaowei ZhangGuo-Chang Fan
Nov 26, 2010·Circulation·Yanjie LuBaofeng Yang
Mar 17, 2011·Journal of Biomedical Science·Bin HeYing-Wei Wang
May 11, 2011·Molecular Biology Reports·Xiaorong HuHong Jiang
Jun 24, 2011·International Journal of Cardiology·Wang-Lin JiangJian Hou
Jan 14, 2012·International Journal of Cardiology·Xiaorong HuHong Jiang
Feb 23, 2012·Cardiovascular Research·Xiaohong WangGuo-Chang Fan
Jan 3, 2013·The Journal of Clinical Investigation·Derek J Hausenloy, Derek M Yellon
Jul 3, 2013·Molecular Cancer Therapeutics·Xuan PanZhao-Xia Wang
Mar 29, 2014·Scandinavian Journal of Immunology·X XuZ Su
Apr 29, 2014·International Journal of Cardiology·Xiaorong HuHong Jiang
May 17, 2014·Lancet·Gerd HeuschLionel Opie
Oct 18, 2014·American Journal of Nephrology·Honglin HuZhenfeng Ye
Nov 25, 2014·Global Cardiology Science & Practice·Georgia KalozoumiDespina Sanoudou
Apr 11, 2015·Journal of the American College of Cardiology·Borja IbáñezFrans Van de Werf
Apr 29, 2016·Molecular Medicine Reports·Jing XieHong Jiang