Abstract
MicroRNAs (miRs) have been demonstrated to be associated with the development, progression and prognosis of gastric cancer. However, the exact role of miR‑27b in the regulation of gastric cancer cells and the underlying mechanisms remain unclear. In the current study, it was demonstrated that miR‑27b was significantly upregulated in gastric cancer tissues and cell lines, compared with their matched normal adjacent tissues and normal gastric epithelial cells, respectively. Luciferase reporter assay data indicated that sprouty2 (SPRY2) is a direct target of miR‑27b, and miR‑27b binds to the 3'‑untranslated region of SPRY2 mRNA. Overexpression of miR‑27b led to a significant reduction in the protein expression of SPRY2, while knockdown of miR‑27b enhanced the SPRY2 protein expression in gastric cancer cells. Furthermore, knockdown of miR‑27b promoted migration and invasion in gastric cancer cells, exhibiting similar effects to those of SPRY2 overexpression on the migration and invasion of gastric cancer cells. Investigation of the molecular mechanisms identified that the activity of extracellular signal‑related kinase (ERK) signaling was mediated by miR‑27b and SPRY2 in gastric cancer cells. In addition, it was observed that SPRY2...Continue Reading
References
Jan 26, 2008·Angiogenesis·Miguel A Cabrita, Gerhard Christofori
May 15, 2009·Oncology·Takeshi TakagiYukihiro Akao
Mar 20, 2010·PLoS Genetics·Jun TieDaiming Fan
Jun 19, 2010·Journal of Cardiovascular Translational Research·Yunhui Cheng, Chunxiang Zhang
Feb 1, 2011·Oncogene·H-J KwakS-H Lee
May 13, 2011·Epigenetics : Official Journal of the DNA Methylation Society·Kwoneel KimYong Sung Kim
Aug 9, 2011·Oncogene·Y XuH Xu
Oct 25, 2011·Cancer Genetics·Ziping ZhangGuangfa Zhao
Dec 31, 2011·Blood·Dauren BiyashevOlga V Volpert
May 24, 2012·Journal of Hepatology·Chunmei WangXin Chen
Nov 16, 2012·Cell Death & Disease·A SacconiG Blandino
Dec 6, 2012·Clinical Pharmacology and Therapeutics·S P Nana-Sinkam, C M Croce
Dec 15, 2012·Journal of Cellular Biochemistry·Yang MeiRobert C H Zhao
Jan 23, 2013·Cancer Research·Lianjin JinSuresh K Alahari
Apr 18, 2013·PloS One·Jun YeJian Huang
Apr 30, 2013·Cell·Luke A YatesRobert J C Gilbert
May 22, 2013·Urology·Pu LiChangjun Yin
Jul 9, 2013·FEBS Letters·Nadine RathmannerHedwig Sutterlüty-Fall
Nov 28, 2013·Oncogene·L ZhangW Xu
Jan 1, 2014·Molecular Cancer Research : MCR·Bo GuoChen Huang
Jan 7, 2014·Molecular and Cellular Biochemistry·Li WanJianjun Wang
Jan 29, 2014·Oncogene·D G R YimD M Virshup
Mar 25, 2014·Journal of Cancer Research and Clinical Oncology·Jiazeng XiaKaiyuan Deng
Jun 8, 2014·Journal of the Chinese Medical Association : JCMA·Chen-Yu KuoChung-Pin Li
Jun 11, 2014·World Journal of Gastroenterology : WJG·Hideyuki IshiguroHiromitsu Takeyama
Jun 14, 2014·Frontiers in Oncology·Christopher M WrightNicole L Simone
Oct 17, 2014·World Journal of Gastroenterology : WJG·Victor PasechnikovMarcis Leja
Nov 6, 2015·Journal of Psychiatric Research·Chieh-Hsin LinHsien-Yuan Lane
Citations
Mar 30, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Li DingJinhai Tang
Mar 11, 2020·Frontiers in Oncology·Huan ZhaoYibo Fan
Jan 14, 2020·BioMed Research International·Yuka MurataKoichiro Wada
Jun 7, 2021·Inflammation·Zhi-Quan XuXiao-Jie He