Upregulation of miR-129-5p affects laryngeal cancer cell proliferation, invasiveness, and migration by affecting STAT3 expression

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Na ShenJun Li

Abstract

microRNA-129-5p may have a relationship with laryngeal squamous cell carcinoma (LSCC), but the expression and function of miRNA-129-5p are not fully understood. This study was performed to investigate the expression and function of miRNA-129-5p in LSCC and its effects on STAT3. HBE and Hep-2 cells were cultured and analyzed by western blotting and real-time PCR. miR-129-5p expression was detected by real-time PCR. Hep-2 cells were transfected with a miR-129-5p expression vector. Cell proliferation, invasion, migration, cell cycle, and apoptosis assays were used to determine the role of miRNA-129-5p in the growth, invasion, and migration of LSCC cells and to determine the importance of STAT3 in these effects. STAT3 mRNA expression in Hep-2 cells was significantly higher than that in HBE cells (P < 0.05). miR-129-5p expression detected by real-time PCR showed that it was expressed at a lower level in Hep-2 cells than in HBE cells (P < 0.05). Compared with the control cells, the transfected cells showed lower STAT3 mRNA expression. For up to 7 days in culture, the transfected cells showed lower proliferation than the control cells (P < 0.05). After 24 h in culture, the apoptosis rate in miR-129-5p-transfected cells was 3.48 ± 0.38...Continue Reading

References

Jun 1, 2002·Science·David S Aaronson, Curt M Horvath
Nov 28, 2002·Gastroenterology·Florian R GretenRoland M Schmid
Sep 17, 2004·Nature·Victor Ambros
Sep 5, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Jung-Hye ChoiIn-Soon Kim
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Jun 19, 2009·Medical Molecular Morphology·Ryo OgawaYoshitaka Fujii
Mar 25, 2010·Biochemical and Biophysical Research Communications·Ruizhe ShenShidan Cheng
Dec 16, 2010·Pediatric Blood & Cancer·Diane K BirksNicholas K Foreman
Apr 13, 2011·Cancer Letters·Valentina MirisolaAngelo Salami
Jul 26, 2011·Biochemical and Biophysical Research Communications·Anita DreherBodil Norrild

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Citations

Feb 13, 2018·Journal of Cellular and Molecular Medicine·Yuling DiaoWenke Zhou
Feb 6, 2020·Experimental and Therapeutic Medicine·Xiaohong LuShiyun Tan
May 27, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Yan-Lin LiHong Wang
Mar 23, 2017·World Journal of Gastroenterology : WJG·Jie WangJian-Xin Jiang
Mar 14, 2018·Cell Death & Disease·Ailiang ZengYongping You
Sep 2, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lin ZhangJie Zhang
Jun 13, 2019·Oncology Letters·Ge LiJiuhui Wang
Aug 10, 2021·Journal of Clinical Laboratory Analysis·Yang XuYuhai Zhang

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