Upregulation of miR-215 exerts neuroprotection effects against ischemic injury via negative regulation of Act1/IL-17RA signaling

Neuroscience Letters
Hongxue SunGuozhong Li

Abstract

This study investigated the role of miR-215 and nuclear factor-κB activator (Act)1 and their mechanisms of action in ischemic stroke. Cell viability was examined with the 3-(4,5-dimethythiazol-. 2-yl)-2,5-diphenyl tetrazolium bromide assay; cell apoptosis was detected by flow cytometry; and mRNA and protein expression was assessed by quantitative real-time PCR and western blotting, respectively. A mouse model of middle cerebral artery occlusion (MCAO) was treated with or without miR-215 mimic to verify the in vitro results. The relationship between miR-215 and interleukin (IL)-17 was evaluated in human peripheral blood from 29 patients. Act1 was upregulated whereas miR-215 was downregulated in ischemic stroke. Overexpression of miR-215 by transfection of a mimic repressed Act1 protein levels in vitro and in vivo, although the luciferase assay showed that miR-215 did not directly bind to the 3' untranslated region of Act1. MiR-215 overexpression inhibited cell apoptosis and autophagy. Increasing miR-215 levels reduced ischemic infarction and improved neurological deficit, while loss of miR-215 phenocopied the effects of IL-17. Upregulation of miR-215 exerts neuroprotection against ischemic injury by negatively regulating Act1/IL...Continue Reading

Citations

Oct 27, 2018·Cell Transplantation·Sheng-Wen WangZhong-Song Shi
Aug 26, 2019·Metabolic Brain Disease·Elaheh HeydariAmir Anbiyaiee
Dec 24, 2019·Reproduction in Domestic Animals = Zuchthygiene·Min YangXiaodong Zhang
Jan 13, 2019·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Nils Henninger, Yunis Mayasi
Apr 23, 2020·Molecular Biology Reports·Fatemeh Javaherforoosh ZadehHadi Rezaeeyan
Sep 17, 2020·Immunology·Qiaohui ZhangYibo Tang

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