Upregulation of P2Y2 receptors by retinoids in normal human epidermal keratinocytes.

Purinergic Signalling
Kayoko FujishitaKazuhide Inoue

Abstract

Retinoids, vitamin A derivatives, are important regulators of the growth and differentiation of skin cells. Although retinoids are therapeutically used for several skin ailments, little is known about their effects on P2 receptors, known to be involved in various functions in the skin. DNA array analysis showed that treatment of normal human epidermal keratinocytes (NHEKs) with all-trans-retinoic acid (ATRA), an agonist to RAR (retinoic acid receptor), enhanced the expression of mRNA for the P2Y2 receptor, a metabotropic P2 receptor that is known to be involved in the proliferation of the epidermis. The expression of other P2 receptors in NHEKs was not affected by ATRA. ATRA increased the mRNA for the P2Y2 receptor in a concentration-dependent fashion (1 nM to 1 muM). Am80, a synthesized agonist to RAR, showed a similar enhancement, whereas 9-cis-retinoic acid (9-cisRA), an agonist to RXR (retinoid X receptor), enhanced P2Y2 gene expression to a lesser extent. Ca(2+) imaging analysis showed that ATRA also increased the function of P2Y2 receptors in NHEKs. Retinoids are known to enhance the turnover of the epidermis by increasing both proliferation and terminal differentiation. The DNA microarray analysis also revealed that ATRA...Continue Reading

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Citations

Nov 3, 2009·Molecular and Cellular Biochemistry·Elsie A OrellanoFernando A González
Dec 7, 2011·Purinergic Signalling·Talita GlaserHenning Ulrich
Mar 29, 2014·Ageing Research Reviews·Márcio LorenciniHoward I Maibach
Dec 14, 2011·The Journal of Investigative Dermatology·Geoffrey BurnstockAina V H Greig

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Methods Mentioned

BETA
PCR

Software Mentioned

Aquacosmos
Primer Express
image
NIH
Microarray Suite Expression Analysis

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