Upregulation of PAWR by small activating RNAs induces cell apoptosis in human prostate cancer cells

Oncology Reports
Kai YangLi-Ping Xie

Abstract

RNA activation (RNAa) is a promising discovery whereby expression of a particular gene can be induced by targeting its promoter using small double-stranded RNAs (dsRNAs) also termed small activating RNAs (saRNAs). We previously reported that several small dsRNAs targeting the PRKC apoptosis WT1 regulator (PAWR) promoter can upregulate PAWR gene expression effectively in human cancer cells. The present study was conducted to evaluate the antitumor potential of PAWR gene induction by these saRNAs in prostate cancer cells. Promisingly, we found that upregulation of PAWR by saRNA inhibited the growth of prostate cancer cells by inducing cell apoptosis which was related to inactivation of the NF-κB and Akt pathways. The decreased anti‑apoptotic protein Bcl-2 and activation of the caspase cascade and poly(ADP-ribose) polymerase (PARP) also supported the efficacy of the treatment. Overall, these data suggest that activation of PAWR by saRNA may have a therapeutic benefit for prostate and other types of cancer.

References

Oct 29, 1998·Genomics·R W JohnstoneY Shi
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·G S Salvesen, V M Dixit
Oct 9, 1999·The Journal of Biological Chemistry·A NalcaV M Rangnekar
Dec 22, 1999·Oncogene·M Barkett, T D Gilmore
Mar 20, 2003·The Journal of Biological Chemistry·Sangeeta K CheemaGabriel Lopez-Berestein
Aug 5, 2003·Molecular and Cellular Biology·Nadia El-GuendyVivek M Rangnekar
Aug 18, 2004·Biochemical Pharmacology·A Ivana Scovassi, Marc Diederich
Jan 20, 2005·Molecular and Cellular Biology·Sushma GurumurthyVivek M Rangnekar
Oct 8, 2005·Molecular Cell·Anindya GoswamiVivek M Rangnekar
Mar 17, 2006·Cancer Research·Anindya GoswamiVivek M Rangnekar
Nov 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Long-Cheng LiRajvir Dahiya
Jan 30, 2007·Nature Chemical Biology·Bethany A JanowskiDavid R Corey
Jul 3, 2007·Cell·Brendan D Manning, Lewis C Cantley
Jan 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Robert F PlaceRajvir Dahiya
Mar 19, 2008·Molecular Cancer Therapeutics·Zhong ChenLong-Cheng Li
Jul 25, 2008·The EMBO Journal·Jayashree JoshiMaria T Diaz-Meco
Jan 23, 2009·Nature·Daniela Castanotto, John J Rossi
Apr 23, 2009·International Journal of Cancer. Journal International Du Cancer·Jared M WhitsonRajvir Dahiya
Jul 25, 2009·Cell Cycle·Maria T Diaz-Meco, Shadi Abu-Baker
Jan 6, 2010·EMBO Molecular Medicine·Katrin Tiemann, John J Rossi
Jan 29, 2010·PloS One·Vera HuangLong-Cheng Li
Sep 14, 2010·Trends in Biotechnology·Monica R LaresDominique L Ouellet
Mar 26, 2013·The Journal of Urology·Tao WangLong-Cheng Li
Apr 16, 2013·The International Journal of Biochemistry & Cell Biology·Kai YangLi-Ping Xie
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal

❮ Previous
Next ❯

Citations

Aug 29, 2016·Clinical & Experimental Metastasis·Reyaz Ur RasoolAnindya Goswami
Sep 19, 2016·Journal of Experimental & Clinical Cancer Research : CR·Huan-Lei WuZhang-Qun Ye
Aug 9, 2020·Tissue Engineering and Regenerative Medicine·Qiaoying ChaiGang Liu
Dec 16, 2020·Cancer Cell International·Jiahong TanQinglei Gao
May 21, 2021·Transplant Immunology·Yao TengQiuling Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis