Upregulation of PPARbeta/delta is associated with structural and functional changes in the type I diabetes rat diaphragm.

PloS One
Nadège SalviCatherine Coirault

Abstract

Diabetes mellitus is associated with alterations in peripheral striated muscles and cardiomyopathy. We examined diaphragmatic function and fiber composition and identified the role of peroxisome proliferator-activated receptors (PPAR alpha and beta/delta) as a factor involved in diaphragm muscle plasticity in response to type I diabetes. Streptozotocin-treated rats were studied after 8 weeks and compared with their controls. Diaphragmatic strips were stimulated in vitro and mechanical and energetic variables were measured, cross bridge kinetics assessed, and the effects of fatigue and hypoxia evaluated. Morphometry, myosin heavy chain isoforms, PPAR alpha and beta/delta gene and protein expression were also assessed. Diabetes induced a decrease in maximum velocity of shortening (-14%, P<0.05) associated with a decrease in myosin ATPase activity (-49%, P<0.05), and an increase in force (+20%, P<0.05) associated with an increase in the number of cross bridges (+14%, P<0.05). These modifications were in agreement with a shift towards slow myosin heavy chain fibers and were associated with an upregulation of PPARbeta/delta (+314% increase in gene and +190% increase in protein expression, P<0.05). In addition, greater resistances to...Continue Reading

References

Dec 1, 1992·Diabetic Medicine : a Journal of the British Diabetic Association·J E WhiteG J Gibson
Jan 1, 1992·Respiration; International Review of Thoracic Diseases·T WankeK Irsigler
Jan 1, 1991·The American Review of Respiratory Disease·T WankeK Irsigler
Apr 1, 1985·Journal of Applied Physiology·J M MetzgerR H Fitts
May 1, 1982·Journal of Cardiovascular Pharmacology·D L BrutsaertE R Van Ocken
Oct 1, 1995·American Journal of Respiratory and Critical Care Medicine·C CoiraultY Lecarpentier
Aug 1, 1994·Journal of Applied Physiology·S Schiaffino, C Reggiani
Apr 1, 1994·Journal of Applied Physiology·C CoiraultY Lecarpentier
Nov 1, 1993·Journal of Applied Physiology·C CoiraultY Lecarpentier
Sep 1, 1993·American Heart Journal·Y LecarpentierD Chemla
Jun 1, 1996·American Journal of Respiratory and Critical Care Medicine·W HidaK Shirato
Jun 1, 1996·Journal of Applied Physiology·G C SieckC E Blanco
Aug 26, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Y LecarpentierC Coirault
Sep 12, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G C SieckK A Jones
Jul 22, 1999·The European Respiratory Journal·C CoiraultY Lecarpentier
Dec 22, 1999·The American Journal of Physiology·C CoiraultY Lecarpentier
Mar 8, 2000·The European Respiratory Journal·P AttalY Lecarpentier
May 18, 2001·Journal of Applied Physiology·T L ClantonN R Prabhakar
Jan 10, 2002·The Journal of Clinical Investigation·Brian N FinckDaniel P Kelly
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Brian N FinckDaniel P Kelly
Apr 23, 2003·Respiratory Physiology & Neurobiology·Erik van Lunteren, Michelle Moyer
Jan 14, 1964·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·A V HILL
Oct 4, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Serge LuquetPaul A Grimaldi
Apr 27, 2004·The Journal of Physiology·Guoxiang YuanNanduri R Prabhakar
Aug 26, 2004·PLoS Biology·Yong-Xu WangRonald M Evans
Jun 5, 2007·Experimental Physiology·Richard A Howlett, Michael C Hogan
Jun 27, 2007·Circulation·Sihem Boudina, E Dale Abel
Nov 6, 2007·Journal of Theoretical Biology·Y LecarpentierC Coirault
Nov 27, 2007·The Journal of Clinical Investigation·Eileen M BurkartDaniel P Kelly
Nov 17, 2009·PloS One·Erik van Lunteren, Michelle Moyer

❮ Previous
Next ❯

Citations

Jun 3, 2014·Critical Care : the Official Journal of the Critical Care Forum·Leigh A Callahan, Gerald S Supinski
Oct 10, 2013·Anesthesiology·Serge CarreiraAlexandre Demoule
Dec 30, 2014·Anesthesiology·Pierre MicheletCatherine Coirault
Nov 7, 2019·Combinatorial Chemistry & High Throughput Screening·Yeqing HuR Zhang
Mar 23, 2017·PloS One·Audrey De JongCatherine Coirault
Apr 4, 2020·Canadian Journal of Physiology and Pharmacology·João Victor Capelli PeixotoFernando Augusto Lavezzo Dias

❮ Previous
Next ❯

Methods Mentioned

BETA
ubiquitination
electrophoresis
PCR

Software Mentioned

NCSS
Image Gauge

Related Concepts

Related Feeds

Autoimmune Diabetes & Tolerance

Patients with type I diabetes lack insulin-producing beta cells due to the loss of immunological tolerance and autoimmune disease. Discover the latest research on targeting tolerance to prevent diabetes.

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.