PMID: 7515577May 11, 1994Paper

Upregulation of secretin receptor gene expression in rat cholangiocytes after bile duct ligation

The American Journal of Physiology
Gianfranco AlpiniN F LaRusso

Abstract

Secretion stimulates ductular bile secretion by binding to receptors on intrahepatic bile duct epithelial cells (i.e., cholangiocytes). In the rat, this choleretic effect increases after bile duct ligation (BDL). Although cholangiocyte proliferation induced by BDL contributes to secretin-induced hypercholeresis, the mechanisms modulating these alterations in secretin-induced ductular bile secretion are obscure. Thus we studied the expression of secretin receptor mRNA (SR-mRNA) in purified liver cells from normal and BDL rats. Northern blot analysis and RNase protection assays with mRNA from purified liver cells demonstrated SR-mRNA only in cholangiocytes; moreover, SR gene expression showed a seven- to ninefold increase in individual cholangiocytes from BDL rats compared with controls. This increase in SR-mRNA expression was related to a similar increase in the rate of transcription of SR-mRNA in cholangiocytes from BDL rats. Thus our studies indicate that 1) SR-mRNA is detected in liver only in cholangiocytes; 2) BDL causes an increase in SR-mRNA in individual cholangiocytes; and 3) the increase in SR-mRNA after BDL is partly related to an increase in the rate of transcription of SR-mRNA by cholangiocytes after BDL. Our data s...Continue Reading

Citations

Jun 16, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·G LesageG Alpini
Jan 24, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gianfranco AlpiniGene LeSage
Apr 1, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Eugenio GaudioGianfranco Alpini
Jun 15, 2006·World Journal of Gastroenterology : WJG·Shannon GlaserGianfranco Alpini
Jun 15, 2006·World Journal of Gastroenterology : WJG·Marco MarzioniAntonio Benedetti
Jan 19, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jin YuJonathan A Dranoff
Sep 18, 2010·The Journal of Veterinary Medical Science·Kazumi TaniguchiKen-ichiro Mutoh
Jul 17, 2013·Experimental Biology and Medicine·Yuyan HanAntonio Franchitto
Oct 3, 2018·International Journal of Molecular Sciences·Debora Maria GiordanoMarco Marzioni
Jun 15, 2006·World Journal of Gastroenterology : WJG·Jesús-M BanalesJuan-F Medina
Nov 9, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Heather L FrancisPaolo Onori
Feb 26, 2009·Expert Reviews in Molecular Medicine·Shannon S GlaserGianfranco Alpini
Dec 24, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kendal JensenShannon Glaser
Oct 20, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anastasia RenziGianfranco Alpini
Jun 26, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shannon GlaserGianfranco Alpini
Sep 12, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Leonardo BaiocchiGianfranco Alpini
Mar 15, 2003·American Journal of Physiology. Cell Physiology·Gianfranco AlpiniTushar Patel
Aug 24, 2001·American Journal of Physiology. Gastrointestinal and Liver Physiology·N KannoG Alpini
Feb 8, 2002·World Journal of Gastroenterology : WJG·Pamela S TietzNicholas F LaRusso
Jul 16, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Anastasia RenziGianfranco Alpini
Jun 30, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Lindsey KennedyGianfranco Alpini
Oct 22, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Luca MarucciGene LeSage
Sep 13, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuyan HanGianfranco Alpini
Apr 27, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nan WuGianfranco Alpini
Jul 7, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Tianhao ZhouGianfranco Alpini
Sep 11, 2019·The Journal of Nutrition·Heather A McCauley
May 19, 2020·International Journal of Peptide Research and Therapeutics·Chiranjib ChakrabortySang-Soo Lee
Jun 15, 2006·World Journal of Gastroenterology : WJG·Eugenio GaudioPaolo Onori
Dec 31, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shannon GlaserGianfranco Alpini
Jun 12, 2004·American Journal of Respiratory Cell and Molecular Biology·Richard J DavisKenneth L Clark
Aug 2, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Nan WuGianfranco Alpini
Mar 22, 2015·The American Journal of Pathology·Debolina RayShannon S Glaser
May 1, 2021·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Lixian ChenGianfranco Alpini
Feb 2, 2011·The American Journal of Pathology·Md Kamruzzaman MunshiShannon S Glaser
Mar 23, 2010·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Shannon GlaserGianfranco Alpini

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