Upregulation of Slit-2 and Slit-3 gene expressions in the nitrofen-induced hypoplastic lung

Journal of Pediatric Surgery
Takashi DoiPrem Puri

Abstract

The pathogenesis of pulmonary hypoplasia in the nitrofen-induced congenital diaphragmatic hernia (CDH) is not clearly understood. Slit-2 and Slit-3 are expressed in fetal lung and play a key role in directing the functional organization and differentiation of lung mesenchyme during branching morphogenesis. We hypothesized that the pulmonary gene expression levels of Slit genes are altered in the nitrofen-induced CDH. Pregnant rats were exposed to either olive oil or nitrofen on day 9 of gestation (D9). Fetal lungs were harvested on D15 and D21 and divided into 2 groups as follows: CDH (n = 9 at each time-point) and control (n = 9 at each time-point). The pulmonary gene expression levels of Slit-2, Slit-3, Robo1, and Robo2 were analyzed by real time reverse transcription polymerase chain reaction. Student's t test or Mann-Whitney U test was used for statistical analysis. Relative messenger RNA expression levels of Slit-2 and Slit-3 were significantly increased in CDH lungs compared to control at both D15 and D21 (P < .05). However, there were no significant differences between CDH and controls in the pulmonary gene expression levels of Robo1 and Robo2 at each time-point. Our results provide evidence, for the first time, that Sli...Continue Reading

References

Mar 8, 2000·Mechanisms of Development·D WarburtonW V Cardoso
Nov 4, 2000·Pediatrics·J Rajagopal, T B Kinane
Jan 10, 2002·Neuron·Kristin L WhitfordAnirvan Ghosh
May 27, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·James M GreenbergAnn L Akeson
Jun 25, 2005·Pediatric Surgery International·A J BennettM Munro
Apr 11, 2008·Pediatric Surgery International·Sandra MontedonicoPrem Puri

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Citations

Feb 18, 2014·Journal of Pediatric Surgery·Florian FriedmacherPrem Puri
May 10, 2011·Revue des maladies respiratoires·A LabbéP-J Déchelotte

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