Upregulation of stromal cell-derived factor 1 (SDF-1) expression in microvasculature endothelial cells in retinal ischemia-reperfusion injury.

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Pinghong LaiShibo Tang

Abstract

Stromal cell-derived factor 1 (SDF-1) is a potent chemotactic and angiogenic factor that has been proposed to play a role in the development of neovascularization. In this study, we explored the expression of SDF-1 in a rat model of retinal ischemia-reperfusion injury and investigated the possible role of retinal microvasculature endothelium cells in generation of this chemokine. Expression patterns of SDF-1 were studied in retina suffering ischemia-reperfusion insult in Sprague-Dawley rats by elevating the intraocular pressure to 110 mm for 60 minutes. The relative level of SDF-1 mRNA in retinas following 6, 12 and 24 hours reperfusion was determined by semi-quantitative RT-PCR. Immunohistochemical methods were used to detect specific lesions expressing SDF-1. The gene expression of SDF-1 in cultured human retinal microvasculature endothelial cells (HRMEC) under hypoxia conditions was assessed by semi-quantitative RT-PCR. The SDF-1 protein was analyzed by immunocytochemistry and fluorescence-activated cell sorting. Upregulation of SDF-1 mRNA (at 6, 12, and 24 hours of reperfusion) was observed, with the expression peak occurring at 12 hours. SDF-1 positive cells appeared initially around the retinal vessels,which diffused into...Continue Reading

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Citations

Jun 25, 2013·Journal of Cellular Biochemistry·Nanmei LiuJinyuan Zhang
Oct 5, 2010·The American Journal of Pathology·Hiroki OtsukaTaiji Sakamoto
Aug 19, 2014·Journal of Hepato-biliary-pancreatic Sciences·Yu SaitoChie Takasu
Jan 22, 2013·Leukemia Research·Rocky PramanikSteven D Mittelman
May 16, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Yeqing WangXiaomei Zhang
Jul 26, 2013·American Journal of Physiology. Renal Physiology·Nanmei LiuJinyuan Zhang
Mar 5, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Laurie D DeLeveLei Wang
Apr 4, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Xinyun KouMing Jin

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