Upregulation of the Chemokine Receptor CCR2B in Epstein‒Barr Virus-Positive Burkitt Lymphoma Cell Lines with the Latency III Program

Viruses
Svetlana KozirevaIrina Kholodnyuk

Abstract

CCR2 is the cognate receptor to the chemokine CCL2. CCR2⁻CCL2 signaling mediates cancer progression and metastasis dissemination. However, the role of CCR2⁻CCL2 signaling in pathogenesis of B-cell malignancies is not clear. Previously, we showed that CCR2B was upregulated in ex vivo peripheral blood B cells upon Epstein‒Barr virus (EBV) infection and in established lymphoblastoid cell lines with the EBV latency III program. EBV latency III is associated with B-cell lymphomas in immunosuppressed patients. The majority of EBV-positive Burkitt lymphoma (BL) tumors are characterized by latency I, but the BL cell lines drift towards latency III during in vitro culture. In this study, the CCR2A and CCR2B expression was assessed in the isogenic EBV-positive BL cell lines with latency I and III using RT-PCR, immunoblotting, and immunostaining analyses. We found that CCR2B is upregulated in the EBV-positive BL cells with latency III. Consequently, we detected the migration of latency III cells toward CCL2. Notably, the G190A mutation, corresponding to SNP CCR2-V64I, was found in one latency III cell line with a reduced migratory response to CCL2. The upregulation of CCR2B may contribute to the enhanced migration of malignant B cells int...Continue Reading

References

May 28, 1992·International Journal of Cancer. Journal International Du Cancer·J RinconM Patarroyo
Feb 23, 2000·Journal of Virology·M SavardJ Gosselin
Feb 20, 2003·Nature Medicine·Sharof M TugizovJoel M Palefsky
Jan 25, 2006·International Journal of Cancer. Journal International Du Cancer·Irina D KholodnyukStefan Imreh
Aug 28, 2007·International Journal of Cancer. Journal International Du Cancer·Maike BuettnerGerald Niedobitek
Jan 24, 2009·International Journal of Cancer. Journal International Du Cancer·Georg W Bornkamm
Jul 18, 2009·Seminars in Cancer Biology·George Klein
Sep 18, 2009·The Journal of Biological Chemistry·Denis SohyJean-Yves Springael
May 8, 2010·Genetics and Molecular Research : GMR·K F NarterT Isbir
May 25, 2010·Biochemical and Biophysical Research Communications·George KleinElena Kashuba
May 10, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Guan-Xian LiuHaihan Song
May 24, 2013·Journal of Virology·Sharof M TugizovJoel M Palefsky
Nov 12, 2014·Annual Review of Pathology·Brian A ZabelEugene C Butcher
Nov 18, 2014·Frontiers in Genetics·Hans H NillerJanos Minarovits
Jan 24, 2015·Experimental & Molecular Medicine·Myung-Soo Kang, Elliott Kieff
Oct 11, 2015·Current Opinion in Virology·Bettina Kempkes, Erle S Robertson
Feb 18, 2016·Oncotarget·Su Yin LimRuth J Muschel
Jun 1, 2016·Frontiers in Immunology·Amanda E I Proudfoot, Mariagrazia Uguccioni
Nov 4, 2016·Nature Reviews. Cancer·Lawrence S YoungPaul G Murray
Feb 9, 2017·International Journal of Molecular Sciences·Martin J StoneJulie Sanchez
Aug 25, 2017·Journal of Virology·Liang Wei WangBenjamin E Gewurz
Sep 13, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Claire Shannon-LoweAndrew I Bell

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Citations

Nov 6, 2018·Medical Microbiology and Immunology·Huali YinRunliang Gan

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Datasets Mentioned

BETA
U937

Methods Mentioned

BETA
PCR
electrophoresis
Assay

Software Mentioned

GraphPad
GraphPad Prism

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