Upregulation of the pro-apoptotic genes BID and FAS in septic shock patients.

Critical Care : the Official Journal of the Critical Care Forum
Fanny Turrel-DavinFabienne Venet

Abstract

Lymphocyte apoptosis has been suggested to play a central role in sepsis pathophysiology, and studies in animal models demonstrated that blocking this pathway improves outcome. However, no routine biomarkers of apoptosis are so far available in patients. Thus, the aim of our study was to assess the different biomarkers of apoptosis putatively usable on a routine basis in septic shock. Thirteen septic shock patients (sampled twice between days 1 to 2 and days 3 to 5 after diagnosis of shock) and 15 sex-matched and age-matched healthy controls were prospectively enrolled. Apoptosis was measured in lymphocyte subpopulations using flow cytometry (Annexin-V binding, activated caspase-3 and Bcl-2 expressions). Representative pro-apoptotic and anti-apoptotic gene expressions were assessed by quantitative reverse-transcription PCR. Monocyte HLA-DR expression and lymphocyte subpopulation cell counts were measured as markers of sepsis-induced immune dysfunctions. To test for statistical significance, the Mann-Whitney U test was used with correction by the number of tests performed. Flow cytometric measurements of apoptosis in septic shock patients showed an increased Annexin-V binding on CD4+ T cells and an increased active caspase-3 exp...Continue Reading

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Citations

Mar 23, 2011·Critical Care : the Official Journal of the Critical Care Forum·Caroline GuignantFabienne Venet
Jun 30, 2011·Critical Care : the Official Journal of the Critical Care Forum·Mary WhiteThomas Ryan
Oct 27, 2011·Critical Care : the Official Journal of the Critical Care Forum·Fanny Turrel-DavinGuillaume Monneret
Jul 20, 2011·Journal of Emergencies, Trauma, and Shock·Fabrizio Giuseppe Bonanno
Dec 14, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Hongmei YuanLi Zhang
Nov 5, 2016·Apoptosis : an International Journal on Programmed Cell Death·Thibaut GirardotGuillaume Monneret
Mar 28, 2013·Anaesthesia and Intensive Care·M HarjaiA B Pant
Aug 13, 2015·Intensive Care Medicine Experimental·David J CainGareth L Ackland

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Methods Mentioned

BETA
PCR
density gradient centrifugation
flow cytometry
reverse-transcription PCR
blood drawing

Software Mentioned

Search
LC
LightCycler

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