Abstract
Ca2+-ATPases of endoplasmic reticulum (SERCAs) are responsible for maintenance of the micro- to millimolar Ca2+ ion concentrations within the endoplasmic reticulum (ER) of eukaryotic cells. This intralumenal Ca2+ storage is important for the generation of Ca2+ signals as well as for the correct folding and posttranslational processing of proteins entering ER after synthesis. ER perturbations such as depletion of Ca2+ or abolishing the oxidative potential, inhibition of glycosylation, or block of secretory pathway, activate the Unfolded Protein Response, consisting of an upregulation of a number of ER-resident chaperones/stress proteins in an effort to boost the impaired folding capacity. We show here that in PC12 cells, depletion of ER Ca2+ by EGTA, as well as inhibition of disulphide bridge formation within the ER by dithiotreitol or inhibition of N-glycosylation by tunicamycin, led to a 2- to 3-fold increase of the SERCA-mediated 45Ca2+ transport to microsomes isolated from cells exposed to these stress agents. The time course of this response corresponded to that for transcriptional upregulation of ER stress proteins, as well as to the increase in the SERCA2b mRNA, as we recently observed in an independent study. These findi...Continue Reading
Citations
Jan 30, 2004·Molecular and Cellular Biology·Branko StefanovicDavid A Brenner
Jun 18, 2009·International Journal of Cancer. Journal International Du Cancer·Luke H StockwinMaja A Bumke
May 23, 2012·The Journal of Infectious Diseases·Carolina BustamanteChristian T Happi
Apr 23, 2004·The Journal of Biological Chemistry·Sic L ChanMark P Mattson
Dec 15, 2010·Molecular Medicine·Wei XinJimei Cai
Apr 4, 2007·Circulation·Sven HorkeUlrich Förstermann
Jun 10, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Estibaliz CastilleroIsaac George
Jun 4, 2019·Acta Physiologica·Wan-Li ChengYi-Jen Chen
May 27, 2020·Journal of Muscle Research and Cell Motility·Nagakatsu HaradaHiroshi Sakaue
Oct 3, 2014·Human Molecular Genetics·Malgorzata ZatykaTimothy G Barrett
Nov 13, 2012·The Journal of Biological Chemistry·Pramod SukumaranKid Törnquist
Aug 13, 2003·The Journal of Cell Biology·Claus ReimertzJochen H M Prehn
Mar 20, 2010·Diabetes·Yunzhou DongMing-Hui Zou
Sep 12, 2019·Cell Death Discovery·Samantha J McDonnellLuminita Paraoan
Jun 20, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Amina KunovacJohn M Hollander
Nov 17, 2020·Frontiers in Cellular Neuroscience·Ina GasterstädtPetra Wahle
Jun 25, 2019·Neurobiology of Disease·Elizabeth R SunderhausDoris Kretzschmar
Apr 10, 2020·Progress in Retinal and Eye Research·Marina S GorbatyukOleg S Gorbatyuk
Mar 21, 2021·European Journal of Nutrition·Aline Fernandes-da-SilvaVanessa Souza-Mello
May 18, 2021·The Journal of Biological Chemistry·Paul F LebeauRichard C Austin
Sep 1, 2004·Biochemical and Biophysical Research Communications·Béla PappChristine Chomienne
Jul 3, 2021·Cancers·Louay BettaiebV'yacheslav Lehen'kyi
Aug 18, 2005·Cell Calcium·Peter VangheluweFrank Wuytack
Dec 9, 2008·Molecular Cell·Mounia ChamiPatrizia Paterlini-Bréchot