Upregulation of the viability of nucleus pulposus cells by bone marrow-derived stromal cells: significance of direct cell-to-cell contact in coculture system

Spine
Yukihiro YamamotoT Hotta

Abstract

Upregulation of the viability of nucleus pulposus cells by coculture with bone marrow-derived stromal cells using a novel culture system. The objective was to apply a novel coculture system having direct cell-to-cell contact between nucleus pulposus cells and bone marrow-derived stromal cells for stimulation of nucleus pulposus cells. Reinsertion of nucleus pulposus cells was effective for treatment of intervertebral disc degeneration. However, obtaining highly viable nucleus pulposus cells was necessary to achieve successful results. Thus, an alternative method to upregulate the biologic and metabolic viabilities of nucleus pulposus cells was desired. Nucleus pulposus cells and bone marrow-derived stromal cells were isolated from New Zealand white rabbits. A 6-well culture plate and insert with track-etched membrane having 0.4 microm pores at the bottom were used for coculture. Nucleus pulposus cells were monocultured, cocultured conventionally (having no direct cell-to-cell contact) with bone marrow-derived stromal cells, or cocultured having direct cell-to-cell contact with bone marrow-derived stromal cells. On day 4 of coculture, nucleus pulposus cells were evaluated for proliferation using WST-8 assay, deoxyribonucleic aci...Continue Reading

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References

Mar 1, 1991·Spine·J P ThompsonD S Bradford
May 15, 1999·Science·B E PetersenJ P Goff
Feb 24, 2001·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M OkumaK Seiki
Apr 5, 2001·Nature·D OrlicP Anversa
Apr 4, 2002·In Vitro Cellular & Developmental Biology. Animal·Toru MizuguchiJacek Rozga
Oct 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Heather A Minges WolsPamela L Witte
Jan 9, 2003·Journal of Clinical Microbiology·D M KuhnM A Ghannoum

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Jul 18, 2006·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Victor Y L LeungKenneth M C Cheung
Nov 14, 2008·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Daisuke Sakai
Jul 16, 2009·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Xinlin Yang, Xudong Li
Dec 1, 2010·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Yun-Tao WangFeng Wang
May 16, 2008·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Fenghua TaoFeng Pan
Apr 21, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Wei LuoFeng Li
May 24, 2007·Tissue Engineering·Damien M O'Halloran, Abhay S Pandit
Sep 3, 2005·Spine·David Greg AndersonAlexander R Vaccaro
May 25, 2006·Spine·Tae-Hong LimSergio A Mendoza
Jan 7, 2014·BMC Veterinary Research·Frances C BachMarianna A Tryfonidou
Jun 3, 2014·The Spine Journal : Official Journal of the North American Spine Society·Tsung-Lin TsaiWan-Ju Li
Nov 5, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Junfen ZengJinwen Zhang
Nov 20, 2013·The Spine Journal : Official Journal of the North American Spine Society·Brian C WernerFrancis H Shen
Sep 2, 2014·Advanced Drug Delivery Reviews·Gianluca FontanaAbhay Pandit
Feb 7, 2013·The Spine Journal : Official Journal of the North American Spine Society·Steven K LeckieJames D Kang
Jun 29, 2012·Journal of Vascular and Interventional Radiology : JVIR·J David PrologoZhenghong Lee
Sep 29, 2011·The Orthopedic Clinics of North America·Daisuke Sakai
May 10, 2011·The International Journal of Biochemistry & Cell Biology·Jin ZhouZhichong Wang
Jan 25, 2005·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Joji Mochida
Jul 7, 2009·Journal of Bioscience and Bioengineering·Dong Hwa KimJung-Woog Shin
Dec 18, 2007·The Spine Journal : Official Journal of the North American Spine Society·Satoshi SobajimaJames D Kang
Nov 21, 2007·The Spine Journal : Official Journal of the North American Spine Society·Gianluca VadalàLars G Gilbertson
Sep 22, 2009·Journal of Internal Medicine·W Richter
Oct 15, 2005·Stem Cells·Stephen M RichardsonJudith A Hoyland
Aug 30, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Masayuki UmedaHirokazu Iida
Dec 13, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Alessandro CorsiPaolo Bianco

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