Abstract
The lack of treatment options other than surgical resection results in unfavourable prognosis of advanced gallbladder carcinoma. The aim of this study was to identify cancer-specific cellular targets that would form the basis for some therapeutic approaches for this disease. Twelve advanced gallbladder carcinoma tissue samples and three samples of normal gallbladder epithelium were screened to identify differentially expressed genes by DNA microarray analysis. The results obtained were validated in an independent sample set by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). Among the genes picked-up, one molecule, topoisomerase IIalpha (TOPO IIalpha), was further assessed immunohistochemically as a potential chemotherapeutic target, and the growth inhibitory effects of etoposide, doxorubicin and idarubicin, representative TOPO IIalpha inhibitors, on two different gallbladder carcinoma cell lines were compared with that of gemcitabine and 5-fulorouracil. Five upregulated genes were identified: four cell cycle-related genes (TOPO IIalpha, cyclin B2, CDC28 protein kinase regulatory subunit 2, ubiquitin-conjugating enzyme E2C) and a metabolism-related gene (gamma-glutamyl hydrolase). Immunohistochem...Continue Reading
References
Dec 1, 1995·Current Opinion in Cell Biology·G I EvanE Harrington
Apr 1, 1996·The British Journal of Surgery·M MiyazakiT Suwa
Mar 1, 1996·Gut·T AjikiY Saitoh
Aug 15, 1996·Cancer·D G GuineeW D Travis
Dec 1, 1996·Current Opinion in Cell Biology·D J Lew, S Kornbluth
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·F M TownsleyJ V Ruderman
Feb 20, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S B SaxmanL H Einhorn
Jun 25, 1998·Molecular and Cellular Biology·E A Egan, M J Solomon
Aug 10, 2000·Journal of Neurosurgery·D YinT Kokunai
Aug 24, 2000·American Journal of Clinical Oncology·C W ChoiY H Kim
Apr 18, 2001·Journal of Clinical Pathology·L NakopoulouC Dimopoulos
Jan 16, 2002·Oncogene·Gen Sheng Wu, Zhenhua Ding
Feb 28, 2002·The Journal of Biological Chemistry·Natalie O KarpinichJohn L Farber
Apr 17, 2002·Journal of Hepato-biliary-pancreatic Surgery·J H Donohue
Jun 18, 2002·The Lancet Oncology·Udo KellnerPierre Rudolph
Aug 16, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J TaïebM Ducreux
Mar 8, 2003·The Lancet Oncology·Sanjeev MisraIndra D Sharma
Apr 26, 2003·American Journal of Clinical Oncology·Imtiaz A Malik, Zeba Aziz
Apr 26, 2003·American Journal of Clinical Oncology·Andre M MuradFlavio C Moore
Jun 20, 2003·Chemotherapy·Ming-Hsien LinWu-Chou Su
Aug 14, 2003·British Journal of Cancer·G MacGroganJ M Coindre
Nov 24, 2004·Cancer·Steven R AlbertsLoren K Tschetter
Apr 1, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Jennifer J KnoxMalcolm J Moore
May 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·C-C ChiuK Fang
Dec 13, 2005·Canadian Journal of Gastroenterology = Journal Canadien De Gastroenterologie·Brian H DingleUNKNOWN Cancer Care Ontario's Program in Evidence-Based Care's Gastrointestinal Cancer Disease Site Group
Mar 21, 2006·Journal of Cellular Physiology·Natalie O KarpinichJohn L Farber
Jun 6, 2006·International Journal of Radiation Oncology, Biology, Physics·John K O'ConnorDavid K Gaffney
Citations
Feb 19, 2009·Nucleic Acids Research·Michal StrosSárka Pospísilová
Feb 22, 2011·Cancer Research·Ryan M GobbleSamuel Singer
Nov 1, 2012·North American Journal of Medical Sciences·Ake Andrén-Sandberg
Dec 29, 2011·Annals of Surgical Oncology·Isabela Werneck da CunhaJosé Vassallo
Sep 9, 2008·Nucleic Acids Research·Guisheng Song, Li Wang
Jul 19, 2012·Digestive Diseases and Sciences·Wei WangHui-Zhen Zhang
Jul 20, 2014·Cytotechnology·Yan LiuQuangen Gao
Aug 31, 2012·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Kshitij SrivastavaBalraj Mittal
Oct 21, 2014·Oncogene·T ChenW Zhang
Jan 24, 2019·Molecular Oncology·Tao LiuMuxiang Zhou
May 15, 2009·Histopathology·Giancarlo TronconeAlfredo Fusco
Jun 15, 2010·Cancer Cytopathology·Eliana GuerrieroGiancarlo Troncone
May 16, 2019·Database : the Journal of Biological Databases and Curation·Ying WangXiaoyan Zhang
Jan 5, 2020·Thoracic Cancer·Reiko SakuraiMasanobu Yamada
Dec 30, 2017·Molecular and Clinical Oncology·Asaf ShveroLea Rath-Wolfson
Jun 28, 2017·World Journal of Gastroenterology : WJG·Aarti SharmaAshok Kumar
Nov 3, 2020·Frontiers in Genetics·Indu Khatri, Manoj K Bhasin