PMID: 8955318Dec 1, 1996Paper

Upstream interactions at the lambda pRM promoter are sequence nonspecific and activate the promoter to a lesser extent than an introduced UP element of an rRNA promoter

Journal of Bacteriology
Y TangP L deHaseth

Abstract

The rightward regulatory region of bacteriophage lambda contains two promoters, pRM and pR, which direct the synthesis of nonoverlapping divergent transcripts from start sites 82 bp apart. Each of the two promoters has an upstream (A+T)-rich region (ATR) within the sequence from -40 to -60 where in the rrnB P1 promoter a stretch of 20 (A+T) bp greatly stimulates promoter function. Here we present an investigation of the possible functional significance of pRM's ATR. We determined the effects on RNA polymerase-pRM promoter interaction both of (G+C) substitutions in the ATR and of amino acid substitutions in the alpha subunit, known to affect the upstream interaction. We find small (two- to threefold) effects of selected mutations in the alpha subunit on open complex formation at pRM. However, the (presumably upstream) interactions underlying these effects are sequence nonspecific, as they are not affected by (G+C) substitutions in the ATR. Substitution of the 20-bp UP element of the rrnB P1 promoter between positions -40 and -60 at pRM stimulates open complex formation to a considerably greater extent (5- to 10-fold). Results from kinetic studies indicate that on this construct the UP element mainly accelerates a step subsequent...Continue Reading

References

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Citations

May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·K Fredrick, J D Helmann
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·S T EstremR L Gourse
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·K MurakamiA Ishihama
Jan 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Caroline A DavisRuth M Saecker
Jan 1, 2005·Proceedings of the National Academy of Sciences of the United States of America·Wilma Ross, Richard L Gourse
May 28, 2013·Journal of Bacteriology·Christine B Michalowski, John W Little
Jun 25, 2008·The Journal of Biological Chemistry·Nataliya BarinovaAndrey Kulbachinskiy
Sep 3, 1998·The Journal of Biological Chemistry·J Xu, G B Koudelka
Feb 4, 1999·Biophysical Journal·Y HaradaT Yanagida

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