Upstream tRNA genes are essential for expression of small nuclear and cytoplasmic RNA genes in trypanosomes.

Molecular and Cellular Biology
V NakaarChristian Tschudi

Abstract

An interesting feature of trypanosome genome organization involves genes transcribed by RNA polymerase III. The U6 small nuclear RNA (snRNA), U-snRNA B (the U3 snRNA homolog), and 7SL RNA genes are closely linked with different, divergently oriented tRNA genes. To test the hypothesis that this association is of functional significance, we generated deletion and block substitution mutants of all three small RNA genes and monitored their effects by transient expression in cultured insect-form cells of Trypanosoma brucei. In each case, two extragenic regulatory elements were mapped to the A and B boxes of the respective companion tRNA gene. In addition, the tRNA(Thr) gene, which is upstream of the U6 snRNA gene, was shown by two different tests to be expressed in T. brucei cells, thus confirming its identity as a gene. This association between tRNA and small RNA genes appears to be a general phenomenon in the family Trypanosomatidae, since it is also observed at the U6 snRNA loci in Leishmania pifanoi and Crithidia fasciculata and at the 7SL RNA locus in L. pifanoi. We propose that the A- and B-box elements of small RNA-associated tRNA genes serve a dual role as intragenic promoter elements for the respective tRNA genes and as ext...Continue Reading

References

Sep 1, 1992·Molecular and Cellular Biology·R H MorseR T Simpson
Mar 1, 1992·Molecular and Biochemical Parasitology·S MichaeliE Ullu
May 1, 1985·Cell·A I Lamond, A A Travers
Jan 1, 1988·Annual Review of Biochemistry·E P Geiduschek, G P Tocchini-Valentini
Dec 9, 1988·Nucleic Acids Research·C TschudiE Ullu
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T A Kunkel
Mar 24, 1994·Nature·D A MaslovL Simpson
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·A P FernandesS M Beverley
Feb 15, 1993·European Journal of Biochemistry·I M Willis

❮ Previous
Next ❯

Citations

Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Kabin XieYinong Yang
May 6, 2015·Cellular Microbiology·Lauriane SollelisYvon Sterkers
Jan 24, 2006·Molecular and Biochemical Parasitology·Jennifer B Palenchar, Vivian Bellofatto
Jul 9, 2013·Essays in Biochemistry·Lauren Lui, Todd Lowe
Aug 19, 2006·Molecular and Biochemical Parasitology·Moshe BitonShulamit Michaeli
Nov 18, 2009·BMC Genomics·J Michael Charette, Michael W Gray
May 8, 2015·PLoS Genetics·John B Moldovan, John V Moran
Feb 20, 2010·Journal of Biomedicine & Biotechnology·Santiago Martínez-CalvilloElisa E Figueroa-Angulo
Dec 5, 2006·Gene·C Daniel EllerYork Marahrens
Nov 3, 2007·Gene·Konstantin P GogolevskyDmitri A Kramerov
Oct 7, 2016·Parasitology·Rodrigo Moreno-CamposSantiago Martínez-Calvillo
Aug 23, 2011·Gene·Anastasia P KovalDmitri A Kramerov
Dec 1, 2006·Gene·Yasuo TakashimaHiroaki Kagawa
May 30, 1998·Nucleic Acids Research·A LevitanS Michaeli
Oct 7, 2011·Nucleic Acids Research·Shulamit MichaeliElisabetta Ullu
May 7, 2010·Nucleic Acids Research·Eric AebyAndré Schneider
Nov 2, 2016·Molecular Microbiology·Gabriela Romero-MezaSantiago Martínez-Calvillo
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y x XuS Michaeli
Feb 13, 2007·Memórias do Instituto Oswaldo Cruz·D L AmbrósioR M B Cicarelli
Feb 14, 2018·PloS One·Aygul IshemgulovaVyacheslav Yurchenko
Jun 22, 2006·The Journal of Biological Chemistry·Elisa GuffantiGiorgio Dieci
Mar 3, 2004·The Journal of Biological Chemistry·Qing LiuShulamit Michaeli
Jul 22, 1998·Molecular and Cellular Biology·E SilvaC Tschudi
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Jan-Peter DanielsBill Wickstead
Apr 13, 2004·Eukaryotic Cell·Santiago Martínez-CalvilloPeter J Myler
Jul 2, 2010·The Journal of Biological Chemistry·Itai Dov TkaczShulamit Michaeli
Oct 31, 2006·Eukaryotic Cell·Carole DumasBarbara Papadopoulou
Oct 24, 2007·Eukaryotic Cell·Anish DasVivian Bellofatto
Jun 27, 2019·IUBMB Life·Guillaume HummelLaurence Drouard
May 7, 2019·BioMed Research International·Fiordaliso C Román-CarraroSantiago Martínez-Calvillo
Mar 20, 2001·Molecular and Biochemical Parasitology·A DjikengC Tschudi

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.