Uptake and antitumoral effects of iodine and 6-iodolactone in differentiated and undifferentiated human prostate cancer cell lines

The Prostate
Nuri ArandaBrenda Anguiano

Abstract

Evidence indicates that iodine per se could be implicated in the physiology of several organs that can internalize it. In thyroid and breast cancer, iodine treatments inhibit cell proliferation and induce apoptosis through a direct (mitochondria) and/or indirect effect (iodolipid generation). Here, we determined the uptake of iodide (I(-) ) and iodine (I(2) ), as well as the antiproliferative and apoptotic effects of 6-iodolactone (6-IL) and both forms of iodine in human prostate cells lines. Non-cancerous (RWPE-1) and cancerous (LNCaP, DU-145) cells, as well as nude mice xenotransplanted with DU-145 cells were used as cancer models. Iodine uptake was analyzed with radioactive tracers, transporter expression by qRT-PCR, cell proliferation by blue trypan, apoptosis by enzyme immunoassay or fluorescence, BAX and BCL-2 by western-blot, and caspsase 3 by enzymatic assay. All three cell lines take up both forms of iodine. In RWPE-1 cells, I(-) uptake depends on the Na(+) /I(-) symporter (NIS), whereas it was independent of NIS in LNCaP and DU-145 cells. Antiproliferative effects of iodine and 6-IL were dose and time dependent; RWPE-1 was most sensitive to I(-) and 6-IL, whereas LNCaP was more sensitive to I(2) . In the three cell li...Continue Reading

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Citations

Apr 24, 2013·Thyroid : Official Journal of the American Thyroid Association·Carmen AcevesGuadalupe Delgado
Dec 7, 2013·Molecular Medicine·Paloma Olvera-CaltzontzinBrenda Anguiano
Jul 16, 2014·Prostaglandins & Other Lipid Mediators·Mario Nava-Villalba, Carmen Aceves
Feb 5, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Lisa ThomaszGuillermo J Juvenal
Feb 11, 2015·Thyroid : Official Journal of the American Thyroid Association·Guadalupe DelgadoCarmen Aceves
Sep 8, 2016·Frontiers in Plant Science·Julia Medrano-MacíasAdalberto Benavides-Mendoza
Oct 21, 2018·European Journal of Human Genetics : EJHG·Mariateresa Di StazioPaolo Gasparini
Jul 10, 2019·Anti-cancer Agents in Medicinal Chemistry·Vladimir G BespalovDenis A Baranenko
Aug 13, 2020·Thyroid : Official Journal of the American Thyroid Association·Cristina Maldonado-AraqueGemma Rojo-Martínez
Jan 31, 2021·International Journal of Molecular Sciences·Carmen AcevesEvangelina Delgado-González

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