Uptake and gene expression of naked plasmid DNA in cultured brain microvessel endothelial cells

Biochemical and Biophysical Research Communications
Masaru NakamuraM Hashida

Abstract

Cellular uptake and gene expression of plasmid DNA and its cationic liposome complexes were studied using primary cultures of bovine brain microvessel endothelial cells (BMEC) developed as an in vitro model of the blood-brain barrier. An avid association of naked plasmid DNA with the BMEC monolayer was observed at 37 degreesC, which is comparable to that of the DNA/liposome complex. The cellular association significantly decreased at low temperature (4 degreesC). The binding at 4 degreesC was saturable and significantly inhibited by polyanions involving polyinosinic acid and dextran sulfate, typical ligands for the macrophage scavenger receptors, but not by polycytidylic acid or in the presence of EDTA. Unexpectedly, a significant gene expression in the BMEC was obtained by transfection with naked plasmid DNA although the expression level was lower than that obtained by plasmid DNA/cationic liposome complex. Taken together, cultured capillary endothelial cells derived from the brain are able to take up naked plasmid DNA via a scavenger receptor like-mediated mechanism for polyanions and gene expression in the cells takes place.

References

Feb 1, 1975·Analytical Biochemistry·C Wang, R L Smith
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P LemarchandR G Crystal
Jun 1, 1986·Journal of Neurochemistry·A Baranczyk-KuzmaR T Borchardt
Jan 1, 1987·Annals of the New York Academy of Sciences·K L Audus, R T Borchardt
Jan 1, 1983·Annual Review of Biochemistry·M S Brown, J L Goldstein
Aug 11, 1995·The Journal of Biological Chemistry·J ZabnerM J Welsh
Nov 1, 1993·Journal of Neurochemistry·H E de VriesD D Breimer

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Citations

Oct 30, 2009·The Journal of Pharmacology and Experimental Therapeutics·Feng LiuLeaf Huang
Nov 5, 2002·Annual Review of Pharmacology and Toxicology·A G de BoerP J Gaillard
Sep 2, 2004·Biological & Pharmaceutical Bulletin·Masaru NakamuraMitsuru Hashida
May 27, 2010·Indian Journal of Pharmaceutical Sciences·P SaraswatB P Nagori
Jan 17, 2016·International Journal of Pharmaceutics·Artita SrimaneeÜlo Langel
Sep 13, 2011·Bioelectrochemistry·Mojca PavlinMaša Kandušer
May 2, 2008·Fish & Shellfish Immunology·Tom Christian TonheimRoy Ambli Dalmo
Nov 17, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kruti S SoniTatiana K Bronich
Apr 20, 2002·Journal of Neuroendocrinology·B KisY Ueta
Jun 30, 2006·The Journal of Gene Medicine·H R MellorR Callaghan
May 31, 2007·The Journal of Gene Medicine·Feng LiuLeaf Huang
Jun 8, 2001·Experimental Biology and Medicine·D J CarrB M Gebhardt
Nov 6, 2021·Journal of Molecular Recognition : JMR·Manjeet Harijan, Meenakshi Singh

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