Uptake and intracellular distribution of different types of nanoparticles in primary human myoblasts and myotubes

International Journal of Pharmaceutics
V GuglielmiM Malatesta

Abstract

The use of nanoparticles as drug carriers in the field of skeletal muscle diseases has been poorly addressed and the interaction of nanoparticles with skeletal muscle cells has been investigated almost exclusively on C2C12 murine myoblasts. In this study we investigated the effects poly(lactide-co-glycolide) nanoparticles, mesoporous silica nanoparticles and liposomes, on the viability of primary human myoblasts and analyzed their cellular uptake and intracellular distribution in both primary human myoblasts and myotubes. Our data demonstrate that poly(lactide-co-glycolide) nanoparticles do not negatively affect myoblasts viability, contrarily to mesoporous silica nanoparticles and liposomes that induce a decrease in cell viability at the highest doses and longest incubation time. Poly(lactide-co-glycolide) nanoparticles and mesoporous silica nanoparticles are internalized by endocytosis, poly(lactide-co-glycolide) nanoparticles undergo endosomal escape whereas mesoporous silica nanoparticles always occur within vacuoles. Liposomes were rarely observed within the cells. The uptake of all tested nanoparticles was less prominent in primary human myotubes as compared to myoblasts. Our findings represent the first step toward the c...Continue Reading

Citations

Jul 12, 2019·Journal of Applied Toxicology : JAT·Na Liu, Meng Tang
Jun 3, 2021·International Journal of Molecular Sciences·Manuela CostanzoManuela Malatesta
Aug 11, 2021·Pharmaceutics·Elham RastegariYueh Chien
Nov 9, 2021·Nanomedicine·Franco TacchiClaudio Cabello-Verrugio

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