PMID: 7049241Jun 14, 1982Paper

Uptake and phosphorylation of 2-deoxy-D-glucose by wild-type and single-kinase strains of Saccharomyces cerevisiae

Biochimica Et Biophysica Acta
A Franzusoff, V P Cirillo

Abstract

The role of phosphorylation in sugar transport in baker's yeast was studied using 2-deoxy-D-glucose. In wild-type baker's yeast, 2-deoxy-D-glucose is accumulated as a mixture of the free sugar and several derivatives. Pool labeling experiments, designed to determine the temporal order of appearance of labeled 2-deoxy-D-glucose in the intracellular pools, have confirmed previous reports that 2-deoxy-D-glucose first appears in the sugar phosphate pool. Such results are consistent with a transport associated phosphorylation mechanism. Since wild-type yeasts contain three enzymes which could participate in such a process, hexokinase isozymes PI and PII and glucokinase, pool labeling experiments were carried out with single-kinase mutant strains containing only one of these enzymes. Results similar to those for wild-type strains were obtained for all three single-kinase strains, suggesting that if transport associated phosphorylation does occur in baker's yeast, it is not a function of the specific kinase present in the cell. While the results of the pool labeling experiments are consistent with a transport associated phosphorylation mechanism for 2-deoxy-D-glucose, caution is urged in interpreting the results of experiments with wh...Continue Reading

References

Aug 15, 1978·Analytical Biochemistry·W M Bonner, J D Stedman
Apr 27, 1979·Science·C M AndersonT A Steitz
Dec 1, 1979·Archives of Biochemistry and Biophysics·E RackerJ Telford
May 1, 1975·European Journal of Biochemistry·T BollerA Wiemken
May 26, 1977·Biochimica Et Biophysica Acta·S A Meredith, A H Romano
Aug 1, 1977·Archives of Biochemistry and Biophysics·Z Lobo, P K Maitra
May 21, 1976·Biochimica Et Biophysica Acta·G F FuhrmannA P Theuvenet
Oct 17, 1975·Biochimica Et Biophysica Acta·H T Jaspers, J van Steveninck
Jan 1, 1970·Annual Review of Biochemistry·H R Kaback
Jan 1, 1970·The Biochemical Journal·T NurminenH Suomalainen
Jun 1, 1972·Journal of Bacteriology·M S Christensen, V P Cirillo
Dec 20, 1974·Molecular and Cellular Biochemistry·R Serrano, G Delafuente
Sep 14, 1971·Biochemistry·A H RamelE A Barnard
Jan 3, 1968·Biochimica Et Biophysica Acta·J van Steveninck, E C Dawson
Apr 29, 1968·Biochimica Et Biophysica Acta·J van Steveninck
Nov 5, 1968·Biochimica Et Biophysica Acta·J van Steveninck
Sep 1, 1981·European Journal of Biochemistry·N VisserP Borst
Feb 1, 1959·The Biochemical Journal·M BURGERA KLEINZELLER
Sep 1, 1962·Journal of Bacteriology·V P CIRILLO
Nov 1, 1965·The Journal of General Physiology·J van Steveninck, A Rothstein

❮ Previous
Next ❯

Citations

Jan 1, 1985·Folia Microbiologica·A KotykH Sychrová
Jan 1, 1986·The International Journal of Biochemistry·A B JenkinsE W Kraegen
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L F Bisson, D G Fraenkel
May 1, 1996·European Journal of Biochemistry·B U StambukR Serrano
Jan 13, 1988·Biochimica Et Biophysica Acta·J SchuddematJ van Steveninck
Oct 23, 1986·Biochimica Et Biophysica Acta·J SchuddematJ Van Steveninck
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·L F BissonD A Lewis
Mar 6, 2010·Journal of Zhejiang University. Science. B·Lea Atanasova, Irina S Druzhinina
Jun 1, 1993·Journal of Bacteriology·D CliftonD G Fraenkel
Jul 1, 1987·Journal of Bacteriology·J M Lang, V P Cirillo
Jul 1, 1997·Applied and Environmental Microbiology·P J MillardR P Haugland
Sep 1, 1983·Journal of Bacteriology·L F Bisson, D G Fraenkel
Dec 1, 1994·Microbiological Reviews·R A WeusthuisJ P van Dijken

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.