Uptake and protein targeting of fluorescent oxidized phospholipids in cultured RAW 264.7 macrophages.

Biochimica Et Biophysica Acta
Ute StemmerAlbin Hermetter

Abstract

The truncated phospholipids 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphocholine (POVPC) and 1-palmitoyl-2-glutaroyl-sn-glycero-3-phosphocholine (PGPC) are oxidation products of 1-palmitoyl-2-arachidonoyl phosphatidylcholine. Depending on concentration and the extent of modification, these compounds induce growth and death, differentiation and inflammation of vascular cells thus playing a role in the development of atherosclerosis. Here we describe the import of fluorescent POVPC and PGPC analogs into cultured RAW 264.7 macrophages and the identification of their primary protein targets. We found that the fluorescent oxidized phospholipids were rapidly taken up by the cells. The cellular target sites depended on the chemical reactivity of these compounds but not on the donor (aqueous lipid suspension, albumin or LDL). The great differences in cellular uptake of PGPC and POVPC are a direct consequence of the subtle structural differences between both molecules. The former compound (carboxyl lipid) can only physically interact with the molecules in its immediate vicinity. In contrast, the aldehydo-lipid covalently reacts with free amino groups of proteins by forming covalent Schiff bases, and thus becomes trapped in the cell...Continue Reading

References

Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M ShevesM Ottolenghi
Apr 16, 1973·Biochimica Et Biophysica Acta·S Batzri, E D Korn
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K UchidaE R Stadtman
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Oct 14, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·G SubbanagounderJ A Berliner
Jun 28, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K KågedalK Ollinger
Oct 20, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Mertens, P Holvoet
Jul 20, 2002·Blood·Mario MairhoferUlrich Salzer
Oct 23, 2003·The Journal of Biological Chemistry·Ulrike ReissJulie D Saba
Apr 13, 2004·Molecular & Cellular Proteomics : MCP·Steven CarrUNKNOWN Working Group on Publication Guidelines for Peptide and Protein Identification Data
May 29, 2004·Current Opinion in Lipidology·Chu-Huang Chen
Aug 15, 2006·Biochimica Et Biophysica Acta·Gilbert O FruhwirthAlbin Hermetter
Dec 1, 2006·Journal of Lipid Research·Alexandra MoumtziAlbin Hermetter
Dec 28, 2006·Apoptosis : an International Journal on Programmed Cell Death·Lars C HuberOliver Distler
Jun 16, 2007·Biochimica Et Biophysica Acta·Gilbert O FruhwirthAlbin Hermetter
Nov 30, 2007·The Journal of Biological Chemistry·Michael E GreenbergStanley L Hazen
Dec 12, 2007·Journal of Lipid Research·B Gabriel GugiuAndrew D Watson
May 8, 2008·Current Opinion in Lipidology·Hans-Peter Deigner, Albin Hermetter
Aug 30, 2008·Sub-cellular Biochemistry·Gilbert O Fruhwirth, Albin Hermetter
Nov 18, 2008·Journal of Lipid Research·Daniel Steinberg
May 23, 2009·Journal of Cell Science·Salah Abu-HamadVarda Shoshan-Barmatz
Apr 10, 2010·Arteriosclerosis, Thrombosis, and Vascular Biology·Eman Al-ShawafDavid J Beech
May 14, 2010·Biochimica Et Biophysica Acta·Christian CodePaavo K J Kinnunen

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Citations

Sep 11, 2012·Lipids in Health and Disease·Ute StemmerAlbin Hermetter
Apr 10, 2013·Biochimica Et Biophysica Acta·Albin HermetterHimanshu Khandelia
Jun 2, 2015·Chemistry and Physics of Lipids·Claudia RamprechtAlbin Hermetter
Mar 28, 2012·Biochimica Et Biophysica Acta·Ute Stemmer, Albin Hermetter
Jan 29, 2020·Antioxidants & Redox Signaling·Irundika H K DiasHelen R Griffiths
May 6, 2014·Soft Matter·Himanshu KhandeliaMartin Hof
Apr 2, 2021·Frontiers in Endocrinology·Marco Di Gioia, Ivan Zanoni

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Methods Mentioned

BETA
electrophoresis
light microscopy
FCS
fluorescence microscopy
fluorescence
FRET

Software Mentioned

AxioVision
BLAST
EasyWin

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