Uptake and trafficking of liposomes to the endoplasmic reticulum

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
Stephanie PollockNicole Zitzmann

Abstract

Liposomes are vesicular structures consisting of an aqueous core surrounded by a lipid bilayer. Apart from the cytosol and lysosomes, no other intracellular compartment has been successfully targeted using liposomal delivery. Here, we report the development of liposomes capable of specific targeting to the endoplasmic reticulum (ER) and associated membranes. Using competition and inhibitor assays along with confocal microscopy, we have determined that ER liposomes utilize scavenger and low-density lipoprotein receptors for endocytosis and enter cells through a caveolin- and microtubule-dependent mechanism. They traffic intact to the ER, where fusion with the ER membrane occurs after 22-25 min, which was confirmed by fluorescence-dequenching assays. Once inside the ER, tagged lipids intercalate with the ER membrane and are subsequently incorporated into ER-assembling entities, such as the ER-budding viruses hepatitis C virus (HCV) and bovine viral diarrhea virus (BVDV), lipid droplets, and secreted lipoproteins. ER liposomes are superior to cytosolic liposome formulations for the intracellular delivery of aqueous cargo, such as HIV-1 antivirals, and are especially suited for the prolonged delivery of lipids and lipophilic drugs ...Continue Reading

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Citations

Apr 11, 2013·Chemical Reviews·Chad P SatoriEdgar A Arriaga
Sep 22, 2010·Proceedings of the National Academy of Sciences of the United States of America·Stephanie PollockNicole Zitzmann
Oct 17, 2012·Antimicrobial Agents and Chemotherapy·Joanna L MillerNicole Zitzmann
Aug 14, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Baomei WangHuang-Ge Zhang
Aug 7, 2013·Nanomedicine·Pranali P DeshpandeVladimir P Torchilin
Nov 26, 2013·Advanced Drug Delivery Reviews·Swati Biswas, Vladimir P Torchilin
Oct 1, 2013·Drug Delivery and Translational Research·Shruti ChopraGuru V Betageri
Apr 29, 2014·Journal of Drug Targeting·Arehalli S ManjappaRayasa S Ramachandra Murthy
May 3, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·William A WalkerJuan L Vivero-Escoto
May 11, 2010·Trends in Microbiology·Andrew C SayceNicole Zitzmann
Apr 2, 2015·ACS Nano·Ezequiel WexselblattYitzhak Tor
Jun 16, 2015·Nature Reviews. Microbiology·Frederic VigantBenhur Lee
Jul 2, 2011·Journal of Hepatology·Margaret F BassendineR Dermot G Neely
May 8, 2016·Biomaterials·Xiaowei MaXing-Jie Liang
Jan 11, 2018·Tissue Engineering. Part a·Genevieve E MellingAlastair J Sloan
May 7, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Gabriela RomeroSergio E Moya
Sep 29, 2019·International Journal of Molecular Sciences·Laura-Sophie BeierJörg Piontek
Aug 29, 2017·The Journal of Clinical Investigation·Xin RongPeter Tontonoz
Dec 9, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yingying ShiJian You
Aug 3, 2019·European Journal of Medicinal Chemistry·Lucia BiasuttoMario Zoratti
Sep 6, 2020·Chemistry and Physics of Lipids·Jinchao Lou, Michael D Best
Jan 15, 2014·Journal of Cell Science·Herschel S DhekneSven C D van Ijzendoorn
May 1, 2021·Pharmaceutics·Supandeep Singh HallanRita Cortesi
Aug 30, 2021·Advances in Colloid and Interface Science·Bijaideep DuttaP A Hassan

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