Uptake, intra-axonal transport and fate of horseradish peroxidase in embryonic spinal neurons of the chick

The Journal of Comparative Neurology
I W Chu-Wang, R W Oppenheim

Abstract

Horseradish peroxidase (HRP) was injected in ovo into the ventral muscle mass of the hind limb of 5- to 7-day-old chick embryos or into the gastrocnemius muscle of 8- to 18-day embryos and localized histochemically. HRP is extensively incorporated via endocytosis into axonal growth cones or presynaptic terminals in the proximity of the injection site. Much of the tracer is taken up in vesicles and small vacuoles. Most of these are smooth-surfaced and only a few are bristle-coated. A small amount of the tracer is also incorporated into the axon terminal through the openings between the axolemma and an intricate membrane channel. The majority of the tracer-laden vesicles and vacuoles rapidly fuse with one another to become large vacuoles, some of which are transformed into multivesicular bodies (MVBs). In axon shafts, many labeled vacuoles and MVBs are transferred to tubule-like organelles, which appear to be the primary carrier for transporting the tracer back to the cell bodies in the lumbar spinal cord. HRP arrives in the sensory ganglia about 0.5-1 hour earlier than in the motoneurons of the lateral motor column. The maximal rate of the retrograde axoplasmic transport is about 3.5 mm/hour. After arriving in the cell bodies, H...Continue Reading

References

Apr 1, 1976·The Journal of Comparative Neurology·R D Broadwell, M W Brightman
Jan 1, 1976·Annals of the New York Academy of Sciences·J W GriffinW K Engel
Jun 17, 1977·Brain Research·N CorvajaC Zucconi
May 1, 1979·The Journal of Comparative Neurology·R D Broadwell, M W Brightman
Apr 15, 1975·The Journal of Comparative Neurology·V Hamburger
Jul 1, 1975·The Journal of Physiology·L Landmesser, D G Morris
Dec 1, 1970·Brain Research·E G Gray, R A Willis
Apr 1, 1971·The Journal of Comparative Neurology·J SchonbachM Cuénoid
Jan 1, 1971·Zeitschrift für Zellforschung und mikroskopische Anatomie·E KawanaK Akert
Oct 1, 1972·The Journal of Comparative Neurology·J W Hinds
Mar 1, 1973·Experimental Neurology·O Z Sellinger, P D Petiet
Jul 12, 1974·Brain Research·P T Turner, A B Harris
Apr 1, 1969·The Journal of Cell Biology·D S Friend
Jun 1, 1971·The Journal of Cell Biology·K M YamadaN K Wessells
Jul 1, 1972·The Journal of Cell Biology·B CeccarelliA Mauro

❮ Previous
Next ❯

Citations

Jan 11, 2011·Progress in Neurobiology·Christopher S Von Bartheld, Amy L Altick
Jun 19, 1995·The Journal of Comparative Neurology·P G St WeckerP B Farel
Feb 15, 1990·The Journal of Comparative Neurology·D C Gordon, F J Richmond

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.