Uptake of 14C-chlorhexidine diacetate to Escherichia coli and Pseudomonas aeruginosa and its release by azolectin

FEMS Microbiology Letters
K A FitzgeraldA D Russell

Abstract

Uptake of 14C-labelled chlorhexidine diacetate (14C-CHA) by wild-type and envelope mutant strains of Escherichia coli and Pseudomonas aeruginosa was very rapid. Maximum uptake was observed within a contact time of 20 s with no additional binding on increased contact, and was concentration-dependent. In contrast to this rapid binding of 14C-CHA, bactericidal studies revealed that the lethal activity of low concentrations of unlabelled CHA was slow, although higher concentrations had a rapid effect. Comparison of a wild-type strain with its envelope mutants indicated that there was little difference in 14C-CHA uptake, in minimal inhibitory concentrations or in bactericidal activity. Azolectin was found to be an effective neutralising agent of biguanide action, but in in vitro agar tests and in reducing or removing the amount of 14C-CHA taken up by the cells.

References

Nov 1, 1986·The Journal of Applied Bacteriology·N IsmaeelA D Russell
Jun 1, 1969·The Journal of Applied Bacteriology·A Davies, B S Field
Jan 1, 1984·Annual Review of Microbiology·R E Hancock
Aug 1, 1984·The Journal of Applied Bacteriology·P BroxtonP Gilbert
Jan 11, 1984·Biochimica Et Biophysica Acta·T IkedaR A Hann

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Citations

Oct 30, 1999·Journal of Applied Microbiology·U TattawasartA D Russell
May 10, 2002·Journal of Applied Microbiology·J-Y Maillard
Apr 1, 1992·The Journal of Applied Bacteriology·S J HiomJ R Dickinson
Jun 1, 1995·Letters in Applied Microbiology·S J HiomA D Russell
Mar 26, 2013·Medicina oral, patología oral y cirugía bucal·May-Lei MeiLakshman-Perera Samaranayake
Nov 28, 2013·Proceedings of the National Academy of Sciences of the United States of America·Karl A HassanIan T Paulsen
Jan 24, 2013·International Journal of Food Microbiology·Elena Ortega MorenteAntonio Gálvez
Apr 3, 2010·Chemistry and Physics of Lipids·Ivana KomljenovićEdward Sternin
Nov 15, 2006·International Journal of Antimicrobial Agents·S L MooreM Jones
Jun 20, 2014·Clinical Oral Implants Research·Alexis IoannidisPatrick R Schmidlin
Jan 18, 2012·Revista latino-americana de enfermagem·Ana Lucía Noreña Peña, Luis Cibanal Juan

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