PMID: 2493081Feb 1, 1989Paper

Uptake of 75Se-selenite by brush border membrane vesicles from chick duodenum stimulated by vitamin D

The Journal of Nutrition
H M Mykkanen, R H Wasserman

Abstract

Brush border membrane vesicles were isolated from mucosal homogenates of duodena from normal, rachitic and vitamin D-treated rachitic chicks using a discontinuous sucrose gradient, and further purified by glycerol gradient centrifugation. In vitro uptake of 75Se-selenite by purified brush border membrane vesicles was studied using a rapid filtration technique. The time course of 75Se uptake was non-linear; rapid initial binding was followed by a gradual decrease in the rate of uptake until an equilibrium value was reached at 60-120 min. The initial binding at 36 s was not affected by selenite concentration in the incubation buffer, while the fractional rate of uptake between the 36 s and 2 min time periods was clearly lower with 1 mM Se than with 4-100 microM Se. 75Se uptake did not show any dependency on the external Na-gradient, nor could it be inhibited by other anions (arsenate, phosphate). Treatment of rachitic chicks either with cholecalciferol (500 Iu, 72 h) or with 1,25(OH)2-cholecalciferol (0.5 microgram given 16 h prior to isolation of the vesicles) significantly enhanced 75Se uptake. A threefold excess of mannitol in the outside buffer reduced 75Se uptake by vesicles from vitamin D-deficient and D-treated chicks 60% ...Continue Reading

Citations

Oct 20, 2009·Nutrition Research Reviews·Viviana CentenoNori Tolosa de Talamoni
Aug 1, 1992·Journal of Inorganic Biochemistry·S C VendelandP D Whanger
Sep 3, 1991·Biochimica Et Biophysica Acta·N Tolosa de TalamoniR H Wasserman

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