Uptake of Benzoic Acid and Chloro-Substituted Benzoic Acids by Alcaligenes denitrificans BRI 3010 and BRI 6011.

Applied and Environmental Microbiology
C B MiguezR A Macleod

Abstract

The mechanism of uptake of benzoic and 2,4-dichlorobenzoic acid (2,4-DCBA) by Alcaligenes denitrificans BRI 3010 and BRI 6011 and Pseudomonas sp. strain B13, three organisms capable of degrading various isomers of chlorinated benzoic acids, was investigated. In all three organisms, uptake of benzoic acid was inducible. For benzoic acid uptake into BRI 3010, monophasic saturation kinetics with apparent K(infm) and V(infmax) values of 1.4 (mu)M and 3.2 nmol/min/mg of cell dry weight, respectively, were obtained. For BRI 6011, biphasic saturation kinetics were observed, suggesting the presence of two uptake systems for benzoic acid with distinct K(infm) (0.72 and 5.3 (mu)M) and V(infmax) (3.3 and 4.6 nmol/min/mg of cell dry weight) values. BRI 3010 and BRI 6011 accumulated benzoic acid against a concentration gradient by a factor of 8 and 10, respectively. A wide range of structural analogs, at 50-fold excess concentrations, inhibited benzoic acid uptake by BRI 3010 and BRI 6011, whereas with B13, only 3-chlorobenzoic acid was an effective inhibitor. For BRI 3010 and BRI 6011, the inhibition by the structural analogs was not of a competitive nature. Uptake of benzoic acid by BRI 3010 and BRI 6011 was inhibited by KCN, by the proto...Continue Reading

References

Aug 1, 1979·Applied and Environmental Microbiology·K FurukawaA Kamibayashi
Sep 1, 1979·Archives of Microbiology·B F TaylorS Pincus
Dec 1, 1975·Journal of Bacteriology·J E Fein, R A MacLeod
Nov 1, 1991·Applied and Environmental Microbiology·B S HernandezD D Focht
Jan 1, 1990·Journal of Bacteriology·P E GroenewegenJ A de Bont
Jul 1, 1990·Applied and Environmental Microbiology·C A PettigrewG S Sayler
Dec 1, 1990·Applied and Environmental Microbiology·W J Hickey, D D Focht
Apr 1, 1989·Applied and Environmental Microbiology·P AdriaensD D Focht
Nov 1, 1989·Biological Chemistry Hoppe-Seyler·S FetznerF Lingens
Jun 15, 1989·Biochemical and Biophysical Research Communications·S FetznerF Lingens
Apr 1, 1989·Canadian Journal of Microbiology·M SylvestreR Massé
Sep 1, 1985·Journal of Bacteriology·M S ShieldsG S Sayler
Jan 1, 1985·Annual Review of Microbiology·E R Kashket
Feb 1, 1986·Journal of Bacteriology·C S Harwood, J Gibson
Jan 1, 1973·Canadian Journal of Microbiology·M Ahmed, D D Focht
Jan 1, 1974·Archives of Microbiology·E DornH J Knackmuss
Aug 1, 1970·Applied Microbiology·R S Horvath, M Alexander
Oct 30, 1984·Biochemical and Biophysical Research Communications·T S MarksA V Quirk
May 1, 1984·Applied and Environmental Microbiology·R MasséM Sylvestre
Mar 1, 1981·Journal of Bacteriology·M Kihara, R M Macnab
May 1, 1981·Journal of Bacteriology·D K ChatterjeeA M Chakrabarty
May 1, 1980·Journal of Bacteriology·W Reineke, H J Knackmuss
Nov 1, 1984·Applied and Environmental Microbiology·T S MarksA V Quirk

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Citations

Dec 9, 2003·Applied and Environmental Microbiology·A S YuroffW J Hickey
Feb 17, 2007·Journal of Basic Microbiology·David Kamanda NgugiHamadi Iddi Boga
Feb 27, 2007·Microbiology·Muhammad Tausif ChaudhryShuang-Jiang Liu
Jun 26, 2012·Applied and Environmental Microbiology·Ying XuNing-Yi Zhou
Jun 8, 2017·FEMS Microbiology Letters·Alpa ChoudharyPrashant S Phale

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