Uptake of bright fluorophore core-silica shell nanoparticles by biological systems

International Journal of Nanomedicine
Andrew ZanePrabir K Dutta

Abstract

Nanoparticles are used in a variety of consumer applications. Silica nanoparticles in particular are common, including as a component of foods. There are concerns that ingested nano-silica particles can cross the intestinal epithelium, enter the circulation, and accumulate in tissues and organs. Thus, tracking these particles is of interest, and fluorescence spectroscopic methods are well-suited for this purpose. However, nanosilica is not fluorescent. In this article, we focus on core-silica shell nanoparticles, using fluorescent Rhodamine 6G, Rhodamine 800, or CdSe/CdS/ZnS quantum dots as the core. These stable fluorophore/silica nanoparticles had surface characteristics similar to those of commercial silica particles. Thus, they were used as model particles to examine internalization by cultured cells, including an epithelial cell line relevant to the gastrointestinal tract. Finally, these particles were administered to mice by gavage, and their presence in various organs, including stomach, small intestine, cecum, colon, kidney, lung, brain, and spleen, was examined. By combining confocal fluorescence microscopy with inductively coupled plasma mass spectrometry, the presence of nanoparticles, rather than their dissolved for...Continue Reading

Citations

Jul 22, 2015·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Christie McCrackenW James Waldman
Oct 31, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·M Di PaoloM L Bossi
Apr 24, 2020·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Ali KermanizadehVicki Stone
Jan 17, 2018·EFSA Journal·UNKNOWN EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS)Claude Lambré
Sep 9, 2019·ACS Biomaterials Science & Engineering·Elizabeth CampbellAnton V Naumov

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Methods Mentioned

BETA
fluorescence microscopy
nuclear magnetic resonance
NMR
transmission electron microscopy
confocal microscopy

Software Mentioned

Malvern

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