PMID: 11322705Apr 27, 2001Paper

Uptake of choline from salmon flesh and its conversion to glycine betaine in response to salt stress in Shewanella putrefaciens

International Journal of Food Microbiology
L LeblancV Pichereau

Abstract

When cultured in M63 minimal medium plus 0.6 M NaCl, the growth of Shewanella putrefaciens was strongly inhibited. The addition of an extract from smoked salmon to this medium restored the growth almost to the unstressed level. A comparison of the 13C NMR spectra of intracellular solutes extracted from S. putrefaciens cells cultured in both conditions revealed the accumulation of glycine betaine (GB) from the smoked salmon extract (SSE). Analysis of the osmoprotective properties of this extract for several strains of Escherichia coli (which differ from each other in their ability to accumulate GB (i) from the surrounding environment, and (ii) from its hydroxylated precursor choline), demonstrated the absence of GB in the SSE. From the overall results, we inferred that salt-stressed S. putrefaciens cells accumulated GB from choline present in the SSE. Furthermore, the use of [14C]-labeled betaines gave evidence that S. putrefaciens (i) oxidised choline to GB, (ii) accumulated GB as a non-metabolisable osmolyte (up to 1300 nmol (mg dw)(-1) when cultured in a medium containing 0.5 M NaCl and either 1 mM choline or 1 mM GB), and (iii) both choline and GB uptake activities were osmotically upregulated (both activities were increased...Continue Reading

References

Jan 1, 1977·Antonie van Leeuwenhoek·K J van Spreekens
Jun 1, 1990·The Journal of Applied Bacteriology·I M Stenström, G Molin
May 1, 1990·International Journal of Food Microbiology·L GramH H Huss
Jan 1, 1988·Archives of Microbiology·M HeckerL Wölfel
Sep 1, 1981·Canadian Journal of Microbiology·S TesoneA Hurst
Dec 1, 1995·International Journal of Food Microbiology·C GutierrezI R Booth
Nov 1, 1996·International Journal of Food Microbiology·E BorchY Blixt
Nov 1, 1996·International Journal of Food Microbiology·L Gram, H H Huss
May 1, 1998·International Journal of Food Microbiology·F LeroiM Cardinal
May 22, 1998·FEMS Microbiology Letters·G I LucchesiC E Domenech
Jan 1, 1999·Journal of Applied Microbiology·J J JoffraudF Chevalier
May 13, 1999·International Journal of Systematic Bacteriology·K VenkateswaranK H Nealson
May 3, 2000·International Journal of Food Microbiology·V PichereauY Auffray
Jun 1, 2000·Applied and Environmental Microbiology·D S NicholsT A McMeekin

❮ Previous
Next ❯

Citations

Aug 2, 2011·Applied and Environmental Microbiology·Danilo ErcoliniFrancesco Villani
Mar 19, 2005·Journal of Bacteriology·Yongqing LiuJizhong Zhou
Sep 3, 2011·PloS One·Jing SunStephen J Giovannoni
Jul 31, 2013·Environmental Pollution·Behzad MortazaviPatricia A Sobecky
Aug 29, 2018·Journal of Food Science and Technology·Fei LyuLin Liu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.