Uptake of copper from plasma proteins in cells where expression of CTR1 has been modulated

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Theodros Z KidaneMaria C Linder

Abstract

Plasma proteins rather than amino acid chelates are the direct sources of copper for mammalian cells. In continuing studies on the mechanisms by which albumin and transcuprein deliver copper and the potential involvement of CTR1, rates of uptake from these proteins and Cu-histidine were compared in cells with/without CTR1 knockdown or knockout. siRNA knocked down expression of CTR1 mRNA 60-85% in human mammary epithelial and hepatic cell models, but this had little or no effect on uptake of 1 μM Cu(II) attached to pure human albumin or alpha-2-macroglobulin. Mouse embryonic fibroblasts that did/did not express Ctr1 took up Cu(II) bound to albumin about as readily as from the histidine complex at physiological concentrations and by a single saturable process. Uptake from mouse albumin achieved a 2-4-fold higher Vmax (with a lower Km) than from heterologous human albumin. Maximum uptake rates from Cu(I)-histidine were >12-fold higher (with higher Km) than for Cu(II), suggesting mediation by a reductase. The presence of cell surface Cu(II) and Fe(III) reductase activity responding only slightly to dehydroascorbate was verified. Excess Fe(III) inhibited uptake from albumin-Cu(II). Ag(I) also inhibited, but kinetics were not or un-c...Continue Reading

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Citations

Feb 23, 2012·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Theodros Z KidaneMaria C Linder
Jul 26, 2013·Journal of the Royal Society, Interface·Renata BehraBarbara Rothen-Rutishauser
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Jan 18, 2018·Antioxidants & Redox Signaling·Petra HeffeterChristian R Kowol
Jun 4, 2017·The Journal of Endocrinology·Rachel T ScarlCraig S Nunemaker
Nov 30, 2018·Metallomics : Integrated Biometal Science·Ewelina StefaniakWojciech Bal
Jul 17, 2020·International Journal of Molecular Sciences·Maria C Linder
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Dec 29, 2020·American Journal of Physiology. Cell Physiology·Ting-Yu ChinSheau-Huei Chueh
Dec 10, 2016·The Journal of Physical Chemistry. B·Ariel R LevySharon Ruthstein

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