PMID: 9186778May 16, 1997Paper

Uptake of creatine phosphate into heart mitochondria: a leak in the creatine shuttle

Biochimica Et Biophysica Acta
S SobollR Krämer

Abstract

CrP uptake into isolated rat heart mitochondria was studied using silicone oil centrifugation. Further, the involvement of the mitochondrial adenine nucleotide translocase was examined by measuring CrP accumulation in mitochondria in the presence of substrates and inhibitors of the ATP/ADP-carrier and by investigating uptake kinetics in liposomes reconstituted with purified bovine heart adenine nucleotide translocase protein. CrP is accumulated in the matrix space of isolated rat heart mitochondria and mitoplasts. The uptake is inhibited by carboxyatractyloside, a specific inhibitor of the mitochondrial adenine nucleotide translocase, and by ADP, phosphoenolpyruvate, 3-phosphoglycerate and pyrophosphate, compounds which are able to bind to the carrier. It is not inhibited when the mitochondrial membrane potential is decreased. CrP is transported into reconstituted liposomes at a rate which is about 3 orders of magnitude lower than the rate for ATP uptake. The transport is sensitive to temperature change and to carboxyatractyloside. It is concluded that CrP is specifically taken up by heart mitochondria via the mitochondrial adenine nucleotide translocase. The transport in mitochondria in situ is facilitated by the close local a...Continue Reading

References

Oct 17, 1968·European Journal of Biochemistry·E Pfaff, M Klingenberg
Jan 1, 1994·Current Biology : CB·T Wallimann

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Citations

Aug 27, 2005·Mitochondrion·Salvatore PassarellaLidia de Bari
May 19, 2009·Cardiovascular Research·Dmitry B ZorovSteven J Sollott
May 31, 2008·Biochimica Et Biophysica Acta·Martin Klingenberg
Aug 17, 2016·Clinical and Translational Medicine·David KitzenbergLouise E Glover
Jul 14, 2000·Physiological Reviews·M Wyss, R Kaddurah-Daouk
Jan 7, 2003·Journal of Applied Physiology·Jeffrey J BraultRonald L Terjung
Jul 12, 2002·American Journal of Physiology. Endocrinology and Metabolism·Bernd WalzelTheo Wallimann
Aug 31, 2018·Radiation Oncology·Yue ZhouXiao Zhou
Oct 18, 2007·Neurochemical Research·Luisa PerassoMaurizio Balestrino
May 1, 2021·Nutrients·Maurizio Balestrino

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