Uptake of fluorescent gentamicin by peripheral vestibular cells after systemic administration

PloS One
Jian-Ping LiuPeter S Steyger

Abstract

In addition to cochleotoxicity, systemic aminoglycoside pharmacotherapy causes vestibulotoxicity resulting in imbalance and visual dysfunction. The underlying trafficking routes of systemically-administered aminoglycosides from the vasculature to the vestibular sensory hair cells are largely unknown. We investigated the trafficking of systemically-administered gentamicin into the peripheral vestibular system in C56Bl/6 mice using fluorescence-tagged gentamicin (gentamicin-Texas-Red, GTTR) imaged by scanning laser confocal microscopy to determine the cellular distribution and intensity of GTTR fluorescence in the three semicircular canal cristae, utricular, and saccular maculae at 5 time points over 4 hours. Low intensity GTTR fluorescence was detected at 0.5 hours as both discrete puncta and diffuse cytoplasmic fluorescence. The intensity of cytoplasmic fluorescence peaked at 3 hours, while punctate fluorescence was plateaued after 3 hours. At 0.5 and 1 hour, higher levels of diffuse GTTR fluorescence were present in transitional cells compared to hair cells and supporting cells. Sensory hair cells typically exhibited only diffuse cytoplasmic fluorescence at all time-points up to 4 hours in this study. In contrast, non-sensory ...Continue Reading

References

Dec 1, 1991·The Annals of Otology, Rhinology, and Laryngology·B K PauwU Fisch
Nov 1, 1991·Antimicrobial Agents and Chemotherapy·D BeauchampM G Bergeron
Dec 1, 1990·Hearing Research·J C de GrootJ E Veldman
Jan 1, 1985·Archives of Oto-rhino-laryngology·T HayashidaT Kurata
Jan 1, 1971·Archiv für klinische und experimentelle Ohren- Nasen- und Kehlkopfheilkunde·J WersällP G Lundquist
Jan 1, 1993·Experimental Brain Research·J T MarcusG F Smoorenburg
Dec 15, 1995·Brain Research·E Hashino, M Shero
Jun 8, 2000·Annals of the New York Academy of Sciences·J M AranD Dulon
May 8, 2001·Science·J M OgleV Ramakrishnan
Sep 25, 2001·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·F O BlackS C Pesznecker
Aug 29, 2003·Journal of the Association for Research in Otolaryngology : JARO·Shun-Ichi Imamura, Joe C Adams
Jun 22, 1957·British Medical Journal·T CAWTHORNE, D RANGER
Oct 3, 2003·Chembiochem : a European Journal of Chemical Biology·Quentin Vicens, Eric Westhof
Nov 8, 2003·Journal of the Association for Research in Otolaryngology : JARO·P S SteygerC F Dai
Jul 9, 2004·Journal of Neurophysiology·Sapan S DesaiAnna Lysakowski
Jul 9, 2004·Journal of Neurophysiology·Sapan S DesaiAnna Lysakowski
Jul 9, 2004·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·F Owen BlackValerie Stallings
Oct 1, 2004·Journal of Neurophysiology·Timo P HirvonenJohn P Carey
Mar 3, 2005·American Journal of Otolaryngology·Tapan K Bhattacharyya, Arvind Kumar
Jun 1, 2005·Hearing Research·Sigrid E MyrdalPeter S Steyger
Jun 15, 2005·Human Movement Science·Dietmar BastaArne Ernst
Feb 3, 2007·Current Pharmaceutical Design·Erol Selimoglu
Feb 20, 2007·International Journal of Antimicrobial Agents·Matthew E Falagas, Ioannis A Bliziotis
Sep 28, 2007·Journal of the Association for Research in Otolaryngology : JARO·Sofia Lyford-PikeJohn P Carey
Dec 18, 2007·Hearing Research·Chun Fu Dai, Peter S Steyger
Jul 24, 2008·The Annals of Pharmacotherapy·Robert E ArianoDiamond A Kassum
Nov 4, 2008·International Journal of Antimicrobial Agents·Emanuele Durante-MangoniMatthew E Falagas
Mar 4, 2009·Journal of the Association for Research in Otolaryngology : JARO·Qi Wang, Peter S Steyger
Jun 22, 2010·Hearing Research·Qi WangP S Steyger
Jan 1, 2009·Seminars in Hearing·Sherri M JonesG Christopher Gaines
Jan 5, 2011·Audiology & Neuro-otology·Ja-Won KooPeter S Steyger
May 10, 2011·PloS One·Hongzhe LiPeter S Steyger
Jul 26, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Takatoshi KarasawaPeter S Steyger
Feb 23, 2012·Scientific Reports·Hongzhe Li, Peter S Steyger

❮ Previous
Next ❯

Citations

Dec 20, 2016·The Journal of Clinical Investigation·Dale W HaileyDavid W Raible
Jun 27, 2017·Ear and Hearing·Ruth Van HeckeLeen Maes
Dec 19, 2018·Cold Spring Harbor Perspectives in Medicine·Corné J Kros, Peter S Steyger
Sep 5, 2018·Amino Acids·Vera A AlferovaVladimir A Korshun
Dec 29, 2020·Journal of Cellular Physiology·Xiaoqing QianDongdong Ren
Aug 13, 2021·Frontiers in Neuroscience·Pattarawadee PrayuenyongPeter S Steyger
Aug 21, 2021·American Journal of Audiology·Peter S Steyger

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
electron microscopy
reversed phase chromatography

Software Mentioned

Image J
Adobe Photoshop
ImageJ
Photoshop

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Related Papers

Journal of the Association for Research in Otolaryngology : JARO
Qi Wang, Peter S Steyger
Journal of the Association for Research in Otolaryngology : JARO
Peter S SteygerC F Dai
© 2021 Meta ULC. All rights reserved