Uptake of inorganic phosphate is a limiting factor for Saccharomyces cerevisiae during growth at low temperatures

FEMS Yeast Research
Isabel VicentRamón Serrano

Abstract

The fermenting ability of Saccharomyces at low temperatures is crucial for the development of alcoholic beverages, but the key factors for the cold tolerance of yeast are not well known. In this report, we present the results of a screening for genes able to confer cold tolerance by overexpression in a laboratory yeast strain auxotrophic for tryptophan. We identified genes of tryptophan permeases (TAT1 and TAT2), suggesting that the first limiting factor in the growth of tryptophan auxotrophic yeast at low temperatures is tryptophan uptake. This fact is of little relevance to industrial strains which are prototrophic for tryptophan. Then, we screened for genes able to confer growth at low temperatures in tryptophan-rich media and found several genes related to phosphate uptake (PHO84, PHO87, PHO90 and GTR1). This suggests that without tryptophan limitation, uptake of inorganic phosphate becomes the limiting factor. We have found that overexpression of the previously uncharacterized ORF YCR015c/CTO1 increases the uptake of inorganic phosphate. Also, genes involved in ergosterol biosynthesis (NSG2) cause improvement of growth at 10°C, dependent on tryptophan uptake, while the gluconeogenesis gene PCK1 and the proline biosynthesis...Continue Reading

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Citations

Jan 18, 2020·Annual Review of Food Science and Technology·B GibsonV Vidgren
Sep 17, 2019·PLoS Genetics·Caiti S Smukowski HeilMaitreya J Dunham
Jun 26, 2020·Genetics and Molecular Biology·Maíra Pompeu MartinsAntonio Rossi
Mar 5, 2019·Current Drug Discovery Technologies·Saif HameedZeeshan Fatima
May 13, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carlos Anton-PlagaroCesar Roncero

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