Uptake of nicotine and extracellular space markers by isolated rat ganglia in relation to receptor activation

British Journal of Pharmacology
D A BrownC N Scholfield

Abstract

1. Uptake of (3)H-nicotine by isolated rat superior cervical sympathetic (SCG) and nodose (NG) ganglia was measured in vitro. Depolarization of the ganglia by nicotine was measured electrically.2. Nicotine depolarized the SCG but not the NG. The mean ED50 for depolarization was 5.3 x 10(-6)M.3. Both ganglia accumulated nicotine when incubated in 3.1 x 10(-5)M(3)H-nicotine: after 30 min incubation the ratios of tissue to medium concentrations were (mean +/- S.E. of mean): SCG, 3.49 +/- 0.13; NG, 2.50 +/- 0.09.4. Total water contents, estimated by drying to constant weight, were: SCG, 83.8 +/- 0.12%; NG, 80.1 +/- 0.21%. Extracellular spaces, measured as (3)H-mannitol space, were: SCG, 38.8 +/- 1.3; NG, 40.3 +/- 0.8% wet weight. These values were not significantly altered by nicotine.5. Correction for tissue fluid spaces indicated that the ratio of the mean intracellular fluid concentration to the extracellular fluid concentration for (3)H-nicotine at 3.1 x 10(-5)M were: SCG, 7.4; NG, 5.6. The ratios were not altered in any consistent manner on varying the nicotine concentration between 3.1 x 10(-7) and 1.6 x 10(-4)M.6. When the nicotine concentration was sufficiently great (6.2 x 10(-6)M or more) to evoke large SCG depolarization...Continue Reading

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Citations

Jan 1, 1972·Naunyn-Schmiedeberg's Archives of Pharmacology·M I Steinberg, R L Volle
Jan 15, 1974·Naunyn-Schmiedeberg's Archives of Pharmacology·W Haefely
Nov 1, 1975·European Journal of Pharmacology·M D Schechter, P Jellinek
Jan 1, 1982·Pharmacology & Therapeutics·C Su
Jan 1, 1984·Neurochemistry International·L Fumagalli, de R Gabriella
May 1, 1972·British Journal of Pharmacology·D A Brown, C N Scholfield
Mar 1, 1979·British Journal of Pharmacology·D A Brown, M P Caulfield
Jan 1, 1978·Drug Metabolism Reviews·M A Russell, C Feyerabend
Jul 19, 1972·Nature: New Biology·N G Bowery, D A Brown
Dec 1, 1982·British Journal of Pharmacology·A J Higgins, M J Neal
Jan 10, 2020·Annual Review of Pharmacology and Toxicology·David A Brown
Sep 15, 1974·Biochemical Pharmacology·S Adamic

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