Uptake of particulate antigens in a nonmammalian lung: phenotypic and functional characterization of avian respiratory phagocytes using bacterial or viral antigens

The Journal of Immunology : Official Journal of the American Association of Immunologists
Eveline D de GeusLonneke Vervelde

Abstract

Major distinctive features of avian lungs are the absence of draining lymph nodes and alveoli and alveolar macrophages (MPhs). However, a large network of MPhs and dendritic cells (DCs) is present in the mucosa of the larger airways and in the linings of the parabronchi. For the modulation of respiratory tract immune responses, for example, by vaccination, a better understanding of Ag uptake in the chicken respiratory tract is needed. In this study, we provide detailed characterization of APCs in chicken lungs, including their functional in vivo activities as measured by the uptake of fluorescently labeled 1-μm beads that are coated with either LPS or inactivated avian influenza A virus (IAV) mimicking the uptake of bacterial or viral Ag. We identified different subsets of MPhs and DCs in chicken lungs, based on the expression of CD11, activation markers, and DEC205. In vivo uptake of LPS- and IAV-beads resulted in an increased percentage MHC class II(+) (MHC II(+)) cells and in the upregulation of CD40. The uptake of LPS-beads resulted in the upregulation of CD80 and MHC II on the cell surface, suggesting either uptake of LPS- and IAV-beads by different subsets of phagocytic cells or LPS-mediated differential activation. Diffe...Continue Reading

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Citations

Jul 25, 2015·FEMS Microbiology Letters·Rodrigo Guabiraba, Catherine Schouler
Mar 10, 2015·Avian Pathology : Journal of the W.V.P.A·Eveline D de GeusLonneke Vervelde
Jul 28, 2016·Viruses·Neda BarjestehShayan Sharif
Jan 13, 2018·Frontiers in Veterinary Science·Damien GarridoRodrigo Guabiraba
Dec 21, 2018·Avian Pathology : Journal of the W.V.P.A·Andreas AlberLonneke Vervelde
Aug 23, 2020·Veterinary Sciences·Michel B VerwooldeAart Lammers
Apr 18, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thien-Phong Vu ManhPascale Quéré
Mar 17, 2020·Frontiers in Immunology·Robin H G A van den BiggelaarChristine Jansen
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Sep 18, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maarten CoorensHenk P Haagsman
Feb 22, 2020·Cellular and Molecular Life Sciences : CMLS·Yi YangShaobin Shang
May 31, 2016·Developmental and Comparative Immunology·Angelica Van GoorSusan J Lamont

Related Concepts

Differentiation Antigens, Hairy Cell Leukemia
Immune-Associated Antigens
Antigens, Viral
Veiled Cells
Influenza in Birds
Lipopolysaccharides
Lung
Orthomyxovirus Type A, Porcine
Macrophages, Alveolar
Structure of Respiratory Epithelium

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