PMID: 6989396Mar 27, 1980Paper

Uptake of the lipophilic cation dibenzyldimethylammonium into Saccharomyces cerevisiae. Interaction with the thiamine transport system

Biochimica Et Biophysica Acta
P W BartsG W Borst-Pauwels

Abstract

The distribution ratio of the lipophilic cation dibenzyldimethylammonium between the cells of Saccharomyces cerevisiae and the medium appears to reflect changes in the membrane potential in a way that is qualitatively correct: the addition of a proton conductor or of an agent which blocks metabolism causes an apparent depolarization of the cell membrane; monovalent cations cause also a lowering of the equilibrium distribution, whereas the addition of divalent cations results in an increase of the partition ratio. However, uptake of dibenzyldimethylammonium and probably also of other liophilic cations proceeds via the thiamine transport system of the yeast. Dibenzyldimethylammonium transport is inducible, like thiamine transport. A kinetic analysis of the mutual interaction between thiamine and dibenzyldimethylammonium uptake shows that these compounds share a common transport system; moreover, dibenzyldimethylammonium uptake is inhibited complete by thiamine disulfide, a competitive inhibitor of thiamine transport and dibenzyldimethylammonium uptake in a thiamine-transport mutant is reduced considerably. It is concluded that one should be cautious when using lipophilic cations to measure the membrane potential of cells of S. ce...Continue Reading

References

Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S RamosH R Kaback
Sep 19, 1977·Biochimica Et Biophysica Acta·A P TheuvenetG W Borst-Pauwels
Dec 1, 1975·Biochimica Et Biophysica Acta·A IwashimaY Nose
Dec 1, 1975·Biochimica Et Biophysica Acta·J A Hoeberichts, G W Borst-Pauwels
Aug 4, 1970·Biochimica Et Biophysica Acta·L E BakeevaV P Skulachev
Jan 1, 1972·The Journal of Membrane Biology·F M Harold, D Papineau
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H HirataF M Harold
Dec 13, 1973·Biochimica Et Biophysica Acta·A IwashimaY Nose
Mar 15, 1974·Biochimica Et Biophysica Acta·J C Riemersma, E J Alsbach
Jul 1, 1973·Journal of Theoretical Biology·G W Borst-Pauwels
Feb 26, 1974·Biochimica Et Biophysica Acta·C De Cespedes, H N Christensen

Citations

Jan 1, 1984·Progress in Biophysics and Molecular Biology·R J Ritchie
Apr 1, 1982·European Journal of Biochemistry·P de la PeñaP S Lazo
Jan 15, 1986·European Journal of Biochemistry·E ValleS Ramos
Mar 23, 1982·Biochimica Et Biophysica Acta·A W BoxmanG W Borst-Pauwels
Sep 14, 1981·Biochimica Et Biophysica Acta·L Kováĉ, L Vareĉka
Feb 23, 1994·Biochimica Et Biophysica Acta·A Ballarin-DentiH Kuroda
Apr 22, 1981·Biochimica Et Biophysica Acta·V VacataK Sigler
Mar 1, 2008·Nature Reviews. Drug Discovery·Paul D Dobson, Douglas B Kell

Related Concepts

Dibenzyldimethylammonium iodide, 14C-labeled
Quaternary Ammonium Compounds
Dinitrophenols
Iodoacetic Acids
Resting Potentials
Saccharomyces cerevisiae
Thiamine Mononitrate

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