PMID: 6989396Mar 27, 1980Paper

Uptake of the lipophilic cation dibenzyldimethylammonium into Saccharomyces cerevisiae. Interaction with the thiamine transport system

Biochimica Et Biophysica Acta
P W BartsG W Borst-Pauwels

Abstract

The distribution ratio of the lipophilic cation dibenzyldimethylammonium between the cells of Saccharomyces cerevisiae and the medium appears to reflect changes in the membrane potential in a way that is qualitatively correct: the addition of a proton conductor or of an agent which blocks metabolism causes an apparent depolarization of the cell membrane; monovalent cations cause also a lowering of the equilibrium distribution, whereas the addition of divalent cations results in an increase of the partition ratio. However, uptake of dibenzyldimethylammonium and probably also of other liophilic cations proceeds via the thiamine transport system of the yeast. Dibenzyldimethylammonium transport is inducible, like thiamine transport. A kinetic analysis of the mutual interaction between thiamine and dibenzyldimethylammonium uptake shows that these compounds share a common transport system; moreover, dibenzyldimethylammonium uptake is inhibited complete by thiamine disulfide, a competitive inhibitor of thiamine transport and dibenzyldimethylammonium uptake in a thiamine-transport mutant is reduced considerably. It is concluded that one should be cautious when using lipophilic cations to measure the membrane potential of cells of S. ce...Continue Reading

References

Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S RamosH R Kaback
Sep 19, 1977·Biochimica Et Biophysica Acta·A P TheuvenetG W Borst-Pauwels
Oct 3, 1977·Biochimica Et Biophysica Acta·G M Roomans, G W Borst-Pauwels
Feb 20, 1979·Biochimica Et Biophysica Acta·G M RoomansG W Borst-Pauwels
Dec 1, 1975·Biochimica Et Biophysica Acta·A IwashimaY Nose
Apr 1, 1975·Archives of Biochemistry and Biophysics·A Peña
Dec 1, 1975·Biochimica Et Biophysica Acta·J A Hoeberichts, G W Borst-Pauwels
Aug 4, 1970·Biochimica Et Biophysica Acta·L E BakeevaV P Skulachev
Jan 1, 1972·The Journal of Membrane Biology·F M Harold, D Papineau
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H HirataF M Harold
Dec 13, 1973·Biochimica Et Biophysica Acta·A IwashimaY Nose
Mar 15, 1974·Biochimica Et Biophysica Acta·J C Riemersma, E J Alsbach
Jan 1, 1972·The Journal of Membrane Biology·F M Harold, D Papineau
Jul 1, 1973·Journal of Theoretical Biology·G W Borst-Pauwels
Feb 26, 1974·Biochimica Et Biophysica Acta·C De Cespedes, H N Christensen
Feb 2, 1971·Biochimica Et Biophysica Acta·G W Borst-PauwelsJ J Henricks
Sep 1, 1963·Journal of Theoretical Biology·T Rosenberg, W Wilbrandt
Sep 22, 1966·Biochemical and Biophysical Research Communications·J BielawskiA L Lehninger
Mar 1, 1967·The Journal of General Physiology·W M Armstrong, A Rothstein
Sep 1, 1964·The Journal of General Physiology·W M ARMSTRONG, A ROTHSTEIN

❮ Previous
Next ❯

Citations

Jan 1, 1984·Progress in Biophysics and Molecular Biology·R J Ritchie
Apr 1, 1982·European Journal of Biochemistry·P de la PeñaP S Lazo
Jan 15, 1986·European Journal of Biochemistry·E ValleS Ramos
Mar 23, 1982·Biochimica Et Biophysica Acta·A W BoxmanG W Borst-Pauwels
Sep 14, 1981·Biochimica Et Biophysica Acta·L Kováĉ, L Vareĉka
Feb 23, 1994·Biochimica Et Biophysica Acta·A Ballarin-DentiH Kuroda
Apr 22, 1981·Biochimica Et Biophysica Acta·V VacataK Sigler
Mar 1, 2008·Nature Reviews. Drug Discovery·Paul D Dobson, Douglas B Kell
Feb 29, 2000·Biochimica Et Biophysica Acta·A Rodríguez-Navarro
Dec 30, 1981·Biochimica Et Biophysica Acta·A Goffeau, C W Slayman
Dec 1, 1981·Biochimica Et Biophysica Acta·G W Borst-Pauwels
Dec 12, 1991·Biochimica Et Biophysica Acta·K Sigler, M Höfer
Apr 1, 1991·Experimental Parasitology·J A Thissen, C C Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved