PMID: 7931703Oct 1, 1994Paper

Uptake, transport and metabolism of exogenous nucleosides in intestinal epithelial cell cultures

The Journal of Nutrition
Y HeW A Walker

Abstract

The uptake and transport of nucleosides (adenosine, cytidine, guanosine, thymidine, uridine and inosine) were examined in intestinal cell lines (Caco-2 and IEC-6). Well-differentiated Caco-2 cells took up significantly more uridine and thymidine than less-differentiated cells over 24 h. These differences were reflected in the resulting trichloroacetic acid-soluble (nucleotide, nucleoside and nucleobase) pool sizes rather than in differences in nucleic acid incorporation. IEC-6 cells have smaller soluble pools than Caco-2 cells, and the uptake of nucleosides over 60 min reflected this difference. Caco-2 cells transported significantly more thymidine, cytidine, guanosine, adenosine and inosine into acid-soluble pools than IEC-6 cells. Caco-2 cells were grown on filters to examine the transcellular transport of nucleosides and to examine the chemical form in which nucleosides appeared at the basolateral aspect of the cell monolayer. The pattern of transport varied for individual nucleosides. The transport of cytidine and guanosine was significantly greater in the apical-to-basolateral direction than in the opposite direction over 2 h. No purine nucleoside was transported intact across the Caco-2 cell monolayer in either direction,...Continue Reading

Citations

Jul 1, 1997·Biological Trace Element Research·C M DekaneyL A Jaeger
Mar 8, 2003·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·A TheisingerE Scharrer
Mar 8, 2006·The FEBS Journal·Maria G TozziPiero L Ipata
Apr 7, 2007·The Journal of Physiology·Ekaitz Errasti-MurugarrenF Javier Casado
Mar 11, 2010·Journal of Clinical Biochemistry and Nutrition·Hirokazu OhtakiSeiji Shioda
Nov 26, 2015·Bulletin of Experimental Biology and Medicine·N V Senyavina, S A Tonevitskaya
Oct 11, 2013·Drug Metabolism and Pharmacokinetics·Tomomi FurihataKan Chiba
Mar 18, 2017·Science·Yingfeng DengPhilipp E Scherer
Jan 16, 2002·Current Opinion in Clinical Nutrition and Metabolic Care·Josef NeuNan Li
Mar 25, 2010·Journal of Agricultural and Food Chemistry·Ko CattoorArne Heyerick
Jan 7, 2004·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Bente SteffansenSven Frokjaer
Mar 29, 2002·Clinics in Perinatology·Josef Neu, Hilton Bernstein

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